Journal ArticleDOI
Rapid detection and specific differentiation of Salmonella enterica subsp. enterica Enteritidis, Typhimurium and its monophasic variant 4,[5],12:i:- by real-time multiplex PCR.
Reads0
Chats0
TLDR
In conclusion, this work provides diagnostic laboratories a fast and accurate method to monitor these Salmonella serovars in chicken neck skin samples based on a 5-plex real-time PCR assay within 24 h after sampling.About:
This article is published in International Journal of Food Microbiology.The article was published on 2015-01-16. It has received 54 citations till now. The article focuses on the topics: Salmonella enterica subsp. enterica & Salmonella enterica.read more
Citations
More filters
Journal ArticleDOI
Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction.
TL;DR: APC-Cas can identify low numbers of S .
Journal ArticleDOI
The Epidemiology of Monophasic Salmonella Typhimurium.
TL;DR: The large-scale application of whole genome sequencing in the last 10 years has revealed the phylogenetic associations of the bacterium and its antimicrobial resistance (AMR) genes and elucidated the role of this zoonotic pathogen in the spread of AMR.
Journal ArticleDOI
Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR.
Raymond Heymans,Amir Vila,Caroliene A. M. van Heerwaarden,Claudia C. C. Jansen,Greetje A.A. Castelijn,Menno van der Voort,Elisabeth G. Biesta-Peters +6 more
TL;DR: The multiplex qPCR assay developed allows for rapid screening of Salmonella spp.
Journal ArticleDOI
A label-free fluorescent direct detection of live Salmonella typhimurium using cascade triple trigger sequences-regenerated strand displacement amplification and hairpin template-generated-scaffolded silver nanoclusters.
Peng Zhang,Hui Liu,Xiaocheng Li,Suzhen Ma,Shuai Men,Heng Wei,Jingjing Cui,Hongning Wang,Hongning Wang +8 more
TL;DR: An intelligently designed sensor system took creative advantage of triple trigger sequences-regenerated strand displacement amplification and self-protective hairpin template-generated-scaffolded silver nanoclusters (AgNCs) for the first time, providing significantly enhanced fluorescent signals to achieve ultrasensitive detection of live S. typhimurium.
Journal ArticleDOI
Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples.
Alejandro Garrido-Maestu,Sarah Azinheiro,Joana Carvalho,Sara Abalde-Cela,Enrique Carbó-Argibay,Lorena Diéguez,Marek Piotrowski,Yury V. Kolen'ko,Marta Prado +8 more
TL;DR: This system is intended for low-cost, in situ, detection of different pathogens, as the proposed methodology can be extrapolated to different microorganisms.
References
More filters
Journal ArticleDOI
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Stephen A. Bustin,Vladimir Benes,Jeremy A. Garson,Jan Hellemans,Jim F. Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Jo Vandesompele,Carl T. Wittwer,Carl T. Wittwer +12 more
TL;DR: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency.
Journal ArticleDOI
Primer3—new capabilities and interfaces
Andreas Untergasser,Ioana Cutcutache,Triinu Koressaar,Jian Ye,Brant C. Faircloth,Maido Remm,Steven G. Rozen +6 more
TL;DR: Primer3’s current capabilities are described, including more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers.
Journal ArticleDOI
Enhancements and modifications of primer design program Primer3
Triinu Koressaar,Maido Remm +1 more
TL;DR: Several enhancements in the widely used primer design program Primer3 are introduced, including a formula for calculating melting temperature and a salt correction formula that can take into account the effects of divalent cations, which are included in most PCR buffers.
Journal ArticleDOI
EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control), 2015. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2013
TL;DR: The European Food Safety Authority and the European Centre for Disease Prevention and Control have analyzed the information on the occurrence of zoonoses and food-borne outbreaks in 2009 submitted by 27 European Union Member States as mentioned in this paper.
Journal ArticleDOI
Multilocus Sequence Typing as a Replacement for Serotyping in Salmonella enterica
Mark Achtman,John Wain,John Wain,François-Xavier Weill,Satheesh Nair,Satheesh Nair,Zhemin Zhou,Vartul Sangal,Mary G. Krauland,James Hale,Heather Harbottle,Alexandra Uesbeck,Gordon Dougan,Lee H. Harrison,Sylvain Brisse +14 more
TL;DR: It is recommended that Salmonella classification by serotyping should be replaced by MLST or its equivalents as it confounded genetically unrelated isolates and failed to recognize natural evolutionary groupings.
Related Papers (5)
Rapid and Simultaneous Identification of Two Salmonella enterica Serotypes, Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR
Hanan I. Malkawi,Raad Gharaibeh +1 more