J
John Wain
Researcher at University of East Anglia
Publications - 217
Citations - 17099
John Wain is an academic researcher from University of East Anglia. The author has contributed to research in topics: Salmonella typhi & Typhoid fever. The author has an hindex of 65, co-authored 205 publications receiving 15356 citations. Previous affiliations of John Wain include John Radcliffe Hospital & Quadram Institute.
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Journal ArticleDOI
Performance comparison of benchtop high-throughput sequencing platforms
Nicholas J. Loman,Raju Misra,Timothy J. Dallman,Chrystala Constantinidou,Saheer E. Gharbia,John Wain,John Wain,Mark J. Pallen +7 more
TL;DR: The performance of these instruments were compared by sequencing an isolate of Escherichia coli O104:H4, which caused an outbreak of food poisoning in Germany in 2011, and the MiSeq had the highest throughput per run and lowest error rates.
Journal ArticleDOI
Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18
Julian Parkhill,Gordon Dougan,Keith D. James,Nicholas R. Thomson,Derek Pickard,John Wain,Carol Churcher,Karen Mungall,Stephen D. Bentley,Matthew T. G. Holden,Mohammed Sebaihia,Stephen Baker,D. Basham,Karen Brooks,Tracey Chillingworth,Phillippa L. Connerton,A. Cronin,P. Davis,Robert L. Davies,L. Dowd,Nicholas J. White,Jeremy Farrar,Theresa Feltwell,N. Hamlin,Ashraful Haque,Tran Tinh Hien,S. Holroyd,Kay Jagels,Anders Krogh,Torben Larsen,S. Leather,Sharon Moule,Peadar O'Gaora,Christopher M. Parry,Michael A. Quail,Kim Rutherford,Mark Simmonds,Jason Skelton,K. Stevens,Sally Whitehead,Bart Barrell +40 more
TL;DR: The genome sequence is sequenced of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands.
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A study of typhoid fever in five asian countries: disease burden and implications for controls
R. Leon Ochiai,Camilo J. Acosta,M. Carolina Danovaro-Holliday,Dong Baiqing,Sujit K. Bhattacharya,Magdarina D. Agtini,Zulfiqar A Bhutta,Do Gia Canh,Mohammad Ali,Seonghye Shin,John Wain,Anne-Laure Page,M. John Albert,Jeremy Farrar,Remon Abu-Elyazeed,Tikki Pang,Claudia M. Galindo,Lorenz von Seidlein,John D. Clemens +18 more
TL;DR: The incidence of typhoid varied substantially between sites, being high in India and Pakistan, intermediate in Indonesia, and low in China and Viet Nam, and underscore the importance of evidence on disease burden in making policy decisions about interventions to control this disease.
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Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants
Gemma C. Langridge,Minh-Duy Phan,Daniel J. Turner,Tim Perkins,Leopold Parts,Jana K. Haase,Ian G. Charles,Duncan J. Maskell,Sarah Peters,Gordon Dougan,John Wain,Julian Parkhill,A. Keith Turner +12 more
TL;DR: This screen validated the hypothesis that the authors can simultaneously assay every gene in the genome to identify niche-specific essential genes and generate a genome-wide list of candidate essential genes.
Journal ArticleDOI
Multilocus Sequence Typing as a Replacement for Serotyping in Salmonella enterica
Mark Achtman,John Wain,John Wain,François-Xavier Weill,Satheesh Nair,Satheesh Nair,Zhemin Zhou,Vartul Sangal,Mary G. Krauland,James Hale,Heather Harbottle,Alexandra Uesbeck,Gordon Dougan,Lee H. Harrison,Sylvain Brisse +14 more
TL;DR: It is recommended that Salmonella classification by serotyping should be replaced by MLST or its equivalents as it confounded genetically unrelated isolates and failed to recognize natural evolutionary groupings.