Journal ArticleDOI
Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using octadecylsilyl silica.
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TLDR
A rapid procedure for the efficient extraction of prostaglandins, thromboxanes and hydroxy fatty acids from urine, plasma and tissue homogenates has been developed and is reported.About:
This article is published in Prostaglandins.The article was published on 1980-11-01. It has received 611 citations till now. The article focuses on the topics: High-performance liquid chromatography & Elution.read more
Citations
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Journal ArticleDOI
Thematic review series: systems biology approaches to metabolic and cardiovascular disorders. Lipidomics: a global approach to lipid analysis in biological systems.
TL;DR: Lipidomics, integrated with genomics, proteomics, and metabolomics, will contribute toward understanding how lipids function in a biological system and will provide a powerful tool for elucidating the mechanism of lipid-based disease, for biomarker screening, and for monitoring pharmacologic therapy.
Book ChapterDOI
Rapid extraction of arachidonic acid metabolites from biological samples using octadecylsilyl silica.
TL;DR: The following fractions can be obtained after extraction of biological samples with ODS silica, if nonpolar PGs such as 13, 14-dihydro-15-oxo-PGE 2 are to be extracted, petroleum ether should be used.
Journal ArticleDOI
Cyclooxygenase-2 inhibition prevents delayed death of CA1 hippocampal neurons following global ischemia.
Masaki Nakayama,Koichi Uchimura,Raymond L. Zhu,Tetsuya Nagayama,Marie E. Rose,R. Anne Stetler,Peter C. Isakson,Jun Chen,Steven H. Graham +8 more
TL;DR: Results suggest that COX2 activity contributes to CA1 neuronal death after global ischemia, and hippocampal prostaglandin E2 concentrations 24 h after global waschemia were decreased in drug-treated animals compared with vehicle-treated controls.
Journal ArticleDOI
Effects of cocoa powder and dark chocolate on LDL oxidative susceptibility and prostaglandin concentrations in humans
Ying Wan,Joe A. Vinson,Terry D. Etherton,John Proch,Sheryl A. Lazarus,Penny M. Kris-Etherton +5 more
TL;DR: Cocoa powder and dark chocolate may favorably affect cardiovascular disease risk status by modestly reducing LDL oxidation susceptibility, increasing serum total antioxidant capacity and HDL-cholesterol concentrations, and not adversely affecting prostaglandins.
Journal ArticleDOI
The use of aspirin to prevent pregnancy-induced hypertension and lower the ratio of thromboxane A2 to prostacyclin in relatively high risk pregnancies
Eyal Schiff,E Peleg,Mordechai Goldenberg,T Rosenthal,E Ruppin,M Tamarkin,G. Barkai,Gilad Ben-Baruch,I Yahal,J Blankstein +9 more
TL;DR: It is concluded that low daily doses of aspirin taken during the third trimester of pregnancy significantly reduce the incidence of pregnancy-induced hypertension and pre-eclamptic toxemia in women at high risk for these disorders, possibly through the correction of an imbalance between levels of thromboxane and prostacyclin.
References
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Journal ArticleDOI
Prostaglandin Endoperoxides. Novel Transformations of Arachidonic Acid in Human Platelets
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: The almost exclusive transformation of the endoperoxide structure into non-prostaglandin derivatives supports the hypothesis that the end operoxides can participate directly and not by way of the classical prostaglandins in regulation of cell functions.
Journal ArticleDOI
Isolation and properties of intermediates in prostaglandin biosynthesis
D.H. Nugteren,E. Hazelhof +1 more
TL;DR: The finding that prostaglandin R can be converted enzymatically into the biologically inactive prostaglandsin D in good yields, reveals a new pathway in prostag landin metabolism and postulates the key position of prostag Landin R as the first biologically active product formed from essential fatty acids.
Journal ArticleDOI
On the Specificity of the Oxygenation of Unsaturated Fatty Acids Catalyzed by Soybean Lipoxidase
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: The conversion of [13l-3H, 3-14C]8,11,14-eicosatrienoic acid is accompanied by an isotope effect, suggesting that the hydrogen removal occurs as the initial step of the reaction, and only the hydrogen of the l configuration is removed by the enzyme during the conversion.
Journal ArticleDOI
Prostaglandin endoperoxides VII. Novel transformations of arachidonic acid in guinea pig lung
Mats Hamberg,Bengt Samuelsson +1 more
TL;DR: Guinea Pig lung homogenates as well as intact guinea pig lung converted added arachidonic acid predominantly into PHD and HHT, metabolites of the prostaglandin endoperoxide PGG2, and to a lesser extent into the classical prostag landins PGE2 and PGF2α.
Journal ArticleDOI
Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.
TL;DR: Somatostatin was rapidly cleaved in vivo and in vitro to a single product, which probably retains biological activity and is likely to be inactivated once it leaves the circulation.