Journal ArticleDOI
Rapid isoelectric focusing in a vertical polyacrylamide minigel system.
TLDR
The procedure permits the application of larger sample volumes containing more protein than other isoelectric focusing procedures, and provides the additional advantages of slab gels over tube gels for analytical purposes, making it possible to complete a two-dimensional gel in 1 day.About:
This article is published in Analytical Biochemistry.The article was published on 1987-12-01. It has received 403 citations till now. The article focuses on the topics: Isoelectric focusing.read more
Citations
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Journal ArticleDOI
Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics.
Thomas Bertrand,Claude Jolivalt,Pierre Briozzo,Eliane Caminade,Nathalie Joly,Catherine Madzak,Christian Mougin +6 more
TL;DR: This is the first high-resolution structure of a multicopper oxidase complexed to a reducing substrate, and it provides a model for engineering laccases that are either more efficient or with a wider substrate specificity.
Journal ArticleDOI
A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin
Mónica Quiroga,Consuelo Guerrero,Miguel A. Botella,Araceli Barceló,Iraida Amaya,María I. Medina,Francisco J. Alonso,Silvia Milrad de Forchetti,Horacio A. Tigier,Victoriano Valpuesta +9 more
TL;DR: It is proposed that the TPX1 product is involved in the synthesis of lignin and suberin, a tomato peroxidase gene the authors have previously isolated.
Journal ArticleDOI
Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis.
TL;DR: Novel fimbriae were isolated and purified from the human enteropathogen Salmonella enteritidis 27655 and represent the third fimbrial type produced by the enteringopathogen S. enter itidis, and had a unique N-terminal amino acid sequence.
Journal ArticleDOI
Isolation and characterization of three chitinases from Trichoderma harzianum.
Jesús de la Cruz,Antonio Hidalgo‐Gallego,José M. Lora,Tahía Benítez,José Antonio Pintor-Toro,Antonio Llobell +5 more
TL;DR: Three proteins which display chitinase activity were purified from the supernatants of Trichoderma harzianum CECT 2413 grown in minimal medium supplemented with Chitin as the sole carbon source, suggesting that each protein is encoded by a different gene.
Journal ArticleDOI
Water Deficit Triggers Phospholipase D Activity in the Resurrection Plant Craterostigma plantagineum
Wolfgang Frank,Teun Munnik,Katja Kerkmann,Francesco Salamini,Dorothea Bartels,Dorothea Bartels +5 more
TL;DR: Experimental evidence that PLD is involved in the early events of dehydration in the resurrection plant Craterostigma plantagineum is provided and a role for heterotrimeric G proteins in PLD regulation is proposed.
References
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Journal ArticleDOI
Two-dimensional gel electrophoresis of membrane proteins
TL;DR: A high-resolution method for two-dimensional separation of membrane proteins is described, a modification of the one recently described by O'Farrell (1975); about 150 different proteins can be visualized in Escherichia coli or Salmonella typhimurim cell envelopes.
Journal ArticleDOI
Evaluation of isoelectric focusing running conditions during two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis: variation of gel patterns with changing conditions and optimized isoelectric focusing conditions.
TL;DR: The systematic evaluation indicated that optimized patterns were obtained when increased concentrations of NaOH and H3PO4 and run durations of 10,000 V-h or longer were used, and the need for internal analytical consistency is emphasized.
Journal ArticleDOI
Isoelectric focusing in agarose
TL;DR: The use of a new agarose for isoelectric focusing of body fluids and tissue proteins even when significantly greater in size than 1 X 10(6) mol.
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Two-dimensional electrophoresis of plasma proteins without denaturing agents.
TL;DR: A technique of two-dimensional polyacrylamide gel electrophoresis for the separation of plasma proteins is described, which permits the simultaneous estimation of pI's and approximate molecular weights of native proteins on the slab gel.
Journal ArticleDOI
Analytical isoelectric focusing using a high-voltage vertical slab polyacrylamide gel system.
TL;DR: High-voltage vertical slab electrofocusing provides a means for rapid resolution of multiple protein samples using stable pH gradients in ranges near pH 2.5 and pH 10.5 in which difficulties have previously been encountered with regard to atmospheric control and temperature regulation using conventional focusing techniques.