Journal ArticleDOI
Reconstructing x-ray fluorescence microtomograms
TLDR
In this article, a self-consistently estimated attenuation of the fluorescence radiation is used to estimate the attenuation inside the sample, which allows one to reconstruct relative concentrations.Abstract:
X-ray fluorescence microtomography allows one to map element distributions inside a sample with high sensitivity and resolutions in the micrometer range. Quantitative reconstruction of the element concentrations from the fluorescence data requires correction for the attenuation inside the sample. However, the attenuation of the fluorescence radiation is not directly accessible by experiment. The method described self-consistently estimates this attenuation and allows one to reconstruct relative concentrations. This is demonstrated on numerical as well as experimental data. A measure for the quality of the reconstruction is given.read more
Citations
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Journal ArticleDOI
Refractive x-ray lenses
Bruno Lengeler,Christian G. Schroer,Marion Kuhlmann,Boris Benner,Til Florian Gunzler,O. Kurapova,Federico Zontone,Anatoly Snigirev,Irina Snigireva +8 more
TL;DR: Parabolic refractive x-ray lenses as discussed by the authors have been developed at Aachen University and are made of beryllium, boron, aluminium, and silicon.
Journal ArticleDOI
Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography
TL;DR: This investigation identifies a clear path toward in vivo molecular imaging using benchtop XFCT techniques in conjunction with GNPs and other metallic probes, up to two orders of magnitude better than conventional x-ray CT.
Journal ArticleDOI
Mapping the local nanostructure inside a specimen by tomographic small-angle x-ray scattering
Christian G. Schroer,Marion Kuhlmann,Stephan V. Roth,Rainer Gehrke,Norbert Stribeck,A. Almendarez-Camarillo,B. Lengeler +6 more
TL;DR: In this paper, small-angle x-ray scattering is combined with scanning microtomography to reconstruct the smallangle diffraction pattern in the direction of the tomographic rotation axis at each location on a virtual section through a specimen.
Book ChapterDOI
Use of Synchrotron-Based Techniques to Elucidate Metal Uptake and Metabolism in Plants
Géraldine Sarret,E.A.H. Pilon Smits,H. Castillo Michel,Marie-Pierre Isaure,Fang-Jie Zhao,Fang-Jie Zhao,Ryan Tappero +6 more
TL;DR: In this paper, the principle and the basics of data analysis for µXRF imaging and tomography, XAS and micro-Fourier transform infrared spectromicroscopy (µFTIR) are presented.
Journal ArticleDOI
Mapping the chemical states of an element inside a sample using tomographic x-ray absorption spectroscopy
Christian G. Schroer,Marion Kuhlmann,Til Florian Gunzler,Bruno Lengeler,M. Richwin,B. Griesebock,Dirk Lützenkirchen-Hecht,Ronald Frahm,Esther Ziegler,A. Mashayekhi,Dean R. Haeffner,Jan-Dierk Grunwaldt,Alfons Baiker +12 more
TL;DR: Hard x-ray absorption spectroscopy is combined with scanning microtomography to reconstruct full near-edge spectra of an elemental species at each location on an arbitrary virtual section through a sample as mentioned in this paper.
References
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Journal ArticleDOI
A microscope for hard x rays based on parabolic compound refractive lenses
Bruno Lengeler,Christian G. Schroer,M. Richwin,J. Tümmler,Michael Drakopoulos,Anatoly Snigirev,Irina Snigireva +6 more
TL;DR: In this article, refractive x-ray lenses with a parabolic profile are described, similar to glass lenses for visible light, which can operate in the range from 2 to 50 degrees of freedom, allowing for magnifications up to 50.
Journal ArticleDOI
Fluorescent computer tomography: a model for correction of X-ray absorption
TL;DR: In this article, the authors used the absorption density, measured using conventional absorption tomography, to remove absorption effects and compared the resulting corrected reconstructions with the reconstructions degraded by attenuation efforts.
Journal ArticleDOI
X-ray fluorescent computer tomography with synchrotron radiation
G.-F. Rust,J. Weigelt +1 more
TL;DR: In this paper, the authors describe the possibility of a quantitative calculation of the distribution of a nonradioactive element within a selected cross section with nondestructive methods with the help of X-ray fluorescent tomography (XFCT) in order to increase measurement sensitivity.
Journal ArticleDOI
Synchrotron hard x-ray microprobe: Fluorescence imaging of single cells
Sylvain Bohic,Alexandre Simionovici,A. A. Snigirev,Richard Ortega,Guillaume Devès,Dominique Heymann,Christian G. Schroer +6 more
TL;DR: In this paper, the authors used synchrotron-induced x-ray fluorescence (SXRF) in the hard X-ray range to image trace elements in single cells.
Journal ArticleDOI
High resolution tomography with chemical specificity
TL;DR: In this paper, a very fast method of computerized critical absorption tomography featuring ∼ 10 μm spatial resolution and high chemical sensitivity is described, which is especially suited to investigating small samples.
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