Journal ArticleDOI
Recovery and Reusability of ApoUnaG Fluorescence Protein from the Unconjugated Bilirubin Complex Structure.
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TLDR
It is concluded that Cu 2+ ion can be used as an agent for the recovery of ApoUnaG protein via binding with UC-BR molecules through absorbance and emission spectra.Abstract:
This study is the first report on the separation and reusability of ApoUnaG protein, indicating excellent fluorescence response with high affinity and specificity toward unconjugated bilirubin (UC-BR) molecules, from the UnaG-UC-BR complex structure. The fluorescence properties of the UnaG-UC-BR complex (holo-UnaG) are studied by addition of different metal ions to perform possible interactions with holo-UnaG through absorbance and emission spectra. After addition of metal ions, some changes with respect to the type of metal ions are observed in fluorescence intensity of the holo-UnaG. When compared to metal ions, an excellent quenching response is sighted in the presence of Cu2+ ions by binding with UC-BR in the UnaG-UC-BR complex structure. Obtained non-fluorescence holo-UnaG-Cu2+ complex mixture is passed through Ni-NTA agarose to remove the ingredients such as Cu2+, UC-BR and Cu2+-UC-BR coordination complex from holo-UnaG. From the obtained experiments, it is concluded that Cu2+ ion can be used as an agent for the recovery of ApoUnaG protein via binding with UC-BR molecules. Graphical Abstract Recovery and Reusability of ApoUnaG Fluorescence Protein from the Unconjugated Bilirubin Complex Structure.read more
References
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Journal ArticleDOI
A Bilirubin-Inducible Fluorescent Protein from Eel Muscle
Akiko Kumagai,Ryoko Ando,Hideyuki Miyatake,Peter Greimel,Toshihide Kobayashi,Yoshio Hirabayashi,Tomomi Shimogori,Atsushi Miyawaki +7 more
TL;DR: The cloning and characterization of UnaG, a fluorescent protein from Japanese eel, is reported, which will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering.
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TL;DR: Bilirubin adsorption experiments from human plasma were performed in a batch experimental setup and MIP particles showed a negligible loss in the bilirubsorption capacity after five Adsorption-desorption cycles with the same adsorbent.
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Emerging fluorescent protein technologies.
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