Journal ArticleDOI
Redox regulation of fos and jun DNA-binding activity in vitro
TLDR
DNA binding of the Fos-Jun heterodimer was modulated by reduction-oxidation of a single conserved cysteine residue in the DNA-binding domains of the two proteins, suggesting that transcriptional activity mediated by AP-1 binding factors may be regulated by a redox mechanism.Abstract:
The proto-oncogenes c-fos and c-jun function cooperatively as inducible transcription factors in signal transduction processes. Their protein products, Fos and Jun, form a heterodimeric complex that interacts with the DNA regulatory element known as the activator protein-1 (AP-1) binding site. Dimerization occurs via interaction between leucine zipper domains and serves to bring into proper juxtaposition a region in each protein that is rich in basic amino acids and that forms a DNA-binding domain. DNA binding of the Fos-Jun heterodimer was modulated by reduction-oxidation (redox) of a single conserved cysteine residue in the DNA-binding domains of the two proteins. Furthermore, a nuclear protein was identified that reduced Fos and Jun and stimulated DNA-binding activity in vitro. These results suggest that transcriptional activity mediated by AP-1 binding factors may be regulated by a redox mechanism.read more
Citations
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Free Radicals in the Physiological Control of Cell Function
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The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Karen Bedard,Karl-Heinz Krause +1 more
TL;DR: This review summarizes the current state of knowledge of the functions of NOX enzymes in physiology and pathology.
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Function and activation of NF-kappa B in the immune system.
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Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple.
TL;DR: Estimates can be used to more fully understand the redox biochemistry that results from oxidative stress, which hopefully will provide a rationale and understanding of the cellular mechanisms associated with cell growth and development, signaling, and reductive or oxidative stress.
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Nitric oxide as a secretory product of mammalian cells.
TL;DR: How different forms of nitric oxide synthase help confer specificity and diversity on the effects of this remarkable signaling molecule is reviewed.
References
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Pamela J. Mitchell,Robert Tjian +1 more
TL;DR: This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
Journal ArticleDOI
Steroid Receptor Regulated Transcription of Specific Genes and Gene Networks
TL;DR: Information is provided on how to identify the Steroid Receptors, Receptor Binding Sites, and other mechanisms that aid in the identification of the receptors and their locations in the genome.
Journal ArticleDOI
Regulation of inducible and tissue-specific gene expression
TL;DR: A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.