Journal ArticleDOI
Redundant Sources of Wnt Regulate Intestinal Stem Cells and Promote Formation of Paneth Cells
TLDR
Wnt is part of a signaling loop that affects homeostasis of intestinal stem and Paneth cells in mice, and Wnt3 signaling is required for growth and development of organoid cultures, whereas nonepithelial Wnt signals could provide a secondary physiological source of Wnt.About:
This article is published in Gastroenterology.The article was published on 2012-12-01. It has received 554 citations till now. The article focuses on the topics: Paneth cell & Wnt signaling pathway.read more
Citations
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Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials
TL;DR: This review focuses on various potential applications of supramolecular hydrogels as molecular biomaterials, classified by their applications in cell cultures, tissue engineering, cell behavior, imaging, and unique applications of hydrogelators.
Journal ArticleDOI
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Meritxell Huch,Craig Dorrell,Sylvia F. Boj,Johan H. van Es,Vivian S. W. Li,Marc van de Wetering,Toshiro Sato,Toshiro Sato,Karien Hamer,Nobuo Sasaki,Milton J. Finegold,Annelise Haft,Robert G.J. Vries,Markus Grompe,Hans Clevers +14 more
TL;DR: Findings indicate that previous observations concerning Lgr5+ stem cells in actively self-renewing tissues can also be extended to damage-induced stem Cells in a tissue with a low rate of spontaneous proliferation.
Journal ArticleDOI
An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control
TL;DR: The widespread importance of Wnt signaling in driving tissue renewal has been revealed by the identification of Axin2 and Lgr5, genes expressed in cells that are responding to Wnt signals, and this crucial role in stem cell self renewal is reviewed.
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Extracellular matrix: A dynamic microenvironment for stem cell niche
TL;DR: Engineered biomaterials able to mimic the in vivo characteristics of stem cell niche provide suitable in vitro tools for dissecting the different roles exerted by the ECM and its molecular components on stem cell behavior.
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Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration
TL;DR: These exciting new insights into the biology of intestinal stem cells have the potential to accelerate the development of stem cell-based therapies and ameliorate cancer treatments.
References
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Journal ArticleDOI
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Toshiro Sato,Robert G.J. Vries,Hugo J. Snippert,Marc van de Wetering,Nick Barker,Daniel E. Stange,Johan H. van Es,Arie Abo,Pekka Kujala,Peter J. Peters,Hans Clevers +10 more
TL;DR: It is concluded that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
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Identification of stem cells in small intestine and colon by marker gene Lgr5
Nick Barker,Johan H. van Es,Jeroen Kuipers,Pekka Kujala,Maaike van den Born,Miranda Cozijnsen,Andrea Haegebarth,Jeroen Korving,Harry Begthel,Peter J. Peters,Hans Clevers +10 more
TL;DR: The expression pattern of Lgr5 suggests that it marks stem cells in multiple adult tissues and cancers, suggesting that it represents the stem cell of the small intestine and colon.
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Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
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Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Toshiro Sato,Johan H. van Es,Hugo J. Snippert,Daniel E. Stange,Robert G.J. Vries,Maaike van den Born,Nick Barker,Noah F. Shroyer,Marc van de Wetering,Hans Clevers +9 more
TL;DR: It is concluded that Lgr5 stem cells compete for essential niche signals provided by a specialized daughter cell, the Paneth cell, in colon crypts, and co-culturing of sorted stem cells with Paneth cells markedly improves organoid formation.