Regulation of chitinase synthesis in Trichoderma harzianum.
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The production of chitinase by Trichoderma species is of interest in relation to their use in biocontrol and as a source of mycolytic enzymes, and the mode of ch itinase synthesis in this fungus is discussed.Abstract:
Summary: The production of chitinase by Trichoderma species is of interest in relation to their use in biocontrol and as a source of mycolytic enzymes. Fourteen isolates of the genus were screened to identify the most effective producer of chitinase. The best strain for chitinase was Trichoderma harzianum 39.1, and this was selected for study of the regulation of enzyme synthesis. Washed mycelium of T. harzianum 39.1 was incubated with a range of carbon sources. Chitinase synthesis was induced on chitin-containing medium, but repressed by glucose and N-acetylglucosamine. Production of the enzyme was optimal at a chitin concentration of 0·5%, at 28 °C, pH 6·0 and was independent of the age of the mycelium. The synthesis of chitinase was blocked by both 8-hydroxyquinoline and cycloheximide, inhibitors of RNA and protein synthesis, respectively. The mode of chitinase synthesis in this fungus is discussed.read more
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Developments in the Biological Control of Soil-borne Plant Pathogens
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Physiology of microbial degradation of chitin and chitosan
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Isolation and characterization of three chitinases from Trichoderma harzianum.
Jesús de la Cruz,Antonio Hidalgo‐Gallego,José M. Lora,Tahía Benítez,José Antonio Pintor-Toro,Antonio Llobell +5 more
TL;DR: Three proteins which display chitinase activity were purified from the supernatants of Trichoderma harzianum CECT 2413 grown in minimal medium supplemented with Chitin as the sole carbon source, suggesting that each protein is encoded by a different gene.
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TL;DR: Serratia marcescens was found to be the most active organism of 100 tested for the production of chitinase and Enterobacter liquefaciens produced nearly as much enzyme.