Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
Reads0
Chats0
TLDR
This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.Abstract:
Introduction There can be little dispute that cellular proliferation is one of the most fundamental of biological processes.' Nor can there be disagreement over the importance of assessing cellular proliferation in the study of many biological processes: indeed, Leblond2 used such assessment for identifying the three major functional types of cellular populationnamely, static, conditional renewal, and continually renewing. The practice of histopathology involves direct or, more usually, indirect assessment of cellular proliferation (and related phenomena such as differentiation) in many situations.3 It is intended that this brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological materialread more
Citations
More filters
Journal ArticleDOI
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.
TL;DR: The Annexin V assay offers the possibility of detecting early phases of apoptosis before the loss of cell membrane integrity and permits measurements of the kinetics of apoptotic death in relation to the cell cycle.
Journal ArticleDOI
Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms
Peter A. Hall,David A. Levison,A. L. Woods,C. C.-W. Yu,D. B. Kellock,J. A. Watkins,Diana M. Barnes,Cheryl Gillett,Richard Camplejohn,Robin Dover,N. H. Waseem,David P. Lane +11 more
TL;DR: Data suggest that in normal tissues and lymphoid neoplasms, PCNA immunolocalization can be used as an index of cell proliferation, however, in some forms of neoplasia, including breast and gastric cancer and in vitro cell lines, the simple relation between PCNA expression and cell proliferation is lost.
Journal ArticleDOI
Evidence That Human Cardiac Myocytes Divide after Myocardial Infarction
Antonio Paolo Beltrami,Konrad Urbanek,Jan Kajstura,Shaomin Yan,Nicoletta Finato,Rossana Bussani,Bernardo Nadal-Ginard,Silvestri F,Annarosa Leri,Carlo Alberto Beltrami,Piero Anversa +10 more
TL;DR: The results challenge the dogma that the adult heart is a postmitotic organ and indicate that the regeneration of myocytes may be a critical component of the increase in muscle mass of the myocardium.
Journal ArticleDOI
Monoclonal antibody Ki-67: its use in histopathology.
D. C. Brown,K C Gatter +1 more
TL;DR: Monoclonal antibody Ki‐67 is of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial therapies.
Journal ArticleDOI
In situ end-labelling detects DNA strand breaks in apoptosis and other physiological and pathological states
TL;DR: In situ end‐labelling stains cells with the morphological characteristics of apoptosis, and greatly simplifies their identification, and in two model systems, the number of labelled cells parallels thenumber of cells undergoing apoptosis as measured by alternative techniques.
References
More filters
Journal ArticleDOI
Flow cytometric DNA analysis of 199 histologically favourable or unfavourable non-Hodgkin lymphomas.
Tuula Lehtinen,Risto Aine,Matti Lehtinen,Olli-Pekka Kallioniemi,Timo Leino,Tapani Hakala,Pauli Leinikki,Martti Alavaikko +7 more
TL;DR: The flow cytometric DNA analysis revealed characteristic features in the favourable and unfavourable NHLs and may be useful in predicting the clinical outcome of patients.
Journal ArticleDOI
DNA flow cytometry of human breast carcinomas and its relationship to transferrin and epidermal growth factor receptors.
TL;DR: DNA flow cytometry provides a more reliable method of assessing the relationship of such growth factor receptors in breast carcinomas to cell proliferation, than that obtained from more subjective tumour grading criteria.
Journal ArticleDOI
Histological grade, elastosis, DNA ploidy and the response to chemotherapy of breast cancer.
TL;DR: In this paper, the relationship between response to chemotherapy of advanced breast cancer and the histological type, grade, elastosis content and DNA ploidy of the primary tumours was examined using paraffin-embedded tissue derived from 125 patients.
Journal Article
Characterization of the fission yeast cdc10+ protein that is required for commitment to the cell cycle.
V. Simanis,P. Nurse +1 more
TL;DR: Antiserum raised against a beta-galactosidase-cdc10+ fusion protein is used to identify the protein product of the cdc10+ start gene of Schizosaccharomyces pombe, required for progress through the G1 phase of the cell cycle and for activating processes such as the increase in histone mRNA level in preparation for S phase.
Journal ArticleDOI
The utility of Ki67 immunostaining, nuclear organizer region counting, and morphology in the assessment of follicular lymphomas
TL;DR: Unless larger series with longer follow‐up suggest otherwise, there is no indication to use either the AgNOR or Ki67 methods which offer no advantage over the simple and inexpensive Working Formulation or Berard's LNCC counting.