Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
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This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.Abstract:
Introduction There can be little dispute that cellular proliferation is one of the most fundamental of biological processes.' Nor can there be disagreement over the importance of assessing cellular proliferation in the study of many biological processes: indeed, Leblond2 used such assessment for identifying the three major functional types of cellular populationnamely, static, conditional renewal, and continually renewing. The practice of histopathology involves direct or, more usually, indirect assessment of cellular proliferation (and related phenomena such as differentiation) in many situations.3 It is intended that this brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological materialread more
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A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.
TL;DR: The Annexin V assay offers the possibility of detecting early phases of apoptosis before the loss of cell membrane integrity and permits measurements of the kinetics of apoptotic death in relation to the cell cycle.
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Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms
Peter A. Hall,David A. Levison,A. L. Woods,C. C.-W. Yu,D. B. Kellock,J. A. Watkins,Diana M. Barnes,Cheryl Gillett,Richard Camplejohn,Robin Dover,N. H. Waseem,David P. Lane +11 more
TL;DR: Data suggest that in normal tissues and lymphoid neoplasms, PCNA immunolocalization can be used as an index of cell proliferation, however, in some forms of neoplasia, including breast and gastric cancer and in vitro cell lines, the simple relation between PCNA expression and cell proliferation is lost.
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Evidence That Human Cardiac Myocytes Divide after Myocardial Infarction
Antonio Paolo Beltrami,Konrad Urbanek,Jan Kajstura,Shaomin Yan,Nicoletta Finato,Rossana Bussani,Bernardo Nadal-Ginard,Silvestri F,Annarosa Leri,Carlo Alberto Beltrami,Piero Anversa +10 more
TL;DR: The results challenge the dogma that the adult heart is a postmitotic organ and indicate that the regeneration of myocytes may be a critical component of the increase in muscle mass of the myocardium.
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Monoclonal antibody Ki-67: its use in histopathology.
D. C. Brown,K C Gatter +1 more
TL;DR: Monoclonal antibody Ki‐67 is of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial therapies.
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In situ end-labelling detects DNA strand breaks in apoptosis and other physiological and pathological states
TL;DR: In situ end‐labelling stains cells with the morphological characteristics of apoptosis, and greatly simplifies their identification, and in two model systems, the number of labelled cells parallels thenumber of cells undergoing apoptosis as measured by alternative techniques.
References
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The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour.
TL;DR: Author commentary of the process is included to cover anticipation of the patient surge, transport of critical patients, and communication across all agencies--interhospital, local, state, and federal.
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Activation of cdc2 protein kinase during mitosis in human cells: Cell cycle-dependent phosphorylation and subunit rearrangement
Giulio Draetta,David Beach +1 more
TL;DR: In this paper, the authors investigated the role of p34 in the regulation of cell cycle in HeLa cells and found that p34 was associated with p62, a newly identified protein that became phosphorylated in vitro.
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Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis.
TL;DR: Semiquantitative immunocytochemical analysis with anti-PCNA/cyclin antibodies may represent a simple, reproducible, yet powerful technique for the routine analysis of cell kinetic data in alcohol-fixed, paraffin-embedded tissue by the surgical pathologist.
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Growth Kinetics of Tumours
TL;DR: This book reviews a workshop held in Geneva in 1977 to discuss the pathogenesis of human breast cancer in relation to hormonal status, immunologic status and possible viral aetiology, and points to future areas of potential interest in viral and hormonal pathogenesis and host immuno-reactivity.
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Nucleolar organizer regions in lymphomas
John Crocker,Paramjit Nar +1 more
TL;DR: Using a silver staining technique, nucleolar organizer region‐associated proteins (Ag‐NORs) have been studied in paraffin sections of 90 non‐Hodgkin's lymphomas, five palatine tonsils and five ‘reactive’ lymph nodes.