Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
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TLDR
This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.Abstract:
Introduction There can be little dispute that cellular proliferation is one of the most fundamental of biological processes.' Nor can there be disagreement over the importance of assessing cellular proliferation in the study of many biological processes: indeed, Leblond2 used such assessment for identifying the three major functional types of cellular populationnamely, static, conditional renewal, and continually renewing. The practice of histopathology involves direct or, more usually, indirect assessment of cellular proliferation (and related phenomena such as differentiation) in many situations.3 It is intended that this brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological materialread more
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Assessment of the labeling index of cohorts of the anterior pituitary cell population in phenobarbital-treated male rats by a double immunohistochemical technique for bromodeoxyuridine and pituitary hormones.
TL;DR: It is concluded that no detectable effects on individual cohort LIs were induced by treatment with phenobarbital for up to 7 days and that any functional adaptation to treatment was associated with increased hormone release.
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The significance of proliferating cell nuclear antigen (PCNA) expression in cancer of the ampulla of vater in terms of prognosis.
Hiroshi Isozaki,Kunio Okajima,Tadashi Ichinona,Masakazu Tanimura,Shinsho Morita,Yoshi Takada,Takashi Ishibashi,Hitoshi Hara +7 more
TL;DR: The results suggest that the PCNA-positive rate provides a prognostic index for cancer of the ampulla of Vater, with results significantly higher than that in patients without metastasis.
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Proliferative Activity of Hürthle Cell Thyroid Tumours
TL;DR: The combination of three types of cell kinetic markers may provide insight into the still poorly understood relationships among karyological abnormalities, enhanced proliferation speed, clonal expansion and invasiveness of HCT.
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Expression of the proliferating cell nuclear antigen and clinicopathological features in intracranial meningiomas.
TL;DR: It is suggested that the proliferative potential is higher in basal meningiomas than in hemispheric mening iomas, which could reflect high recurrence and difficulty in management of the skull base meninguomas.
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Expression of the cyclin-dependent kinase inhibitor p27 in transitional cell bladder cancers: is it a good predictor for tumor behavior?
TL;DR: In this article, the authors investigated the immunohistochemical expression of p27 intransitional cell bladder cancers and itsrelationship with clinicopathological data,proliferating cell nuclear antigen (PCNA) andp53 oncoprotein immunoreactivity.
References
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Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.
TL;DR: The data suggest that the early stages of mitogen stimulation represent initial sequences of proliferation and not parts of the cell cycle, and immunostaining with monoclonal antibody Ki-67 provides a reliable means of rapidly evaluating the growth fraction of normal and neoplastic human cell populations.
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Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication
TL;DR: Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro and do not cross-react with thymidine.
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Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
TL;DR: A first series of immunostainings of tumour biopsies indicated that Ki‐67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.
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Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry
TL;DR: There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin.
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Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
TL;DR: Cyclin and the auxiliary protein of DNA polymerase-δ are identical, and it is reported here that these two are identical.