Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
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This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.Abstract:
Introduction There can be little dispute that cellular proliferation is one of the most fundamental of biological processes.' Nor can there be disagreement over the importance of assessing cellular proliferation in the study of many biological processes: indeed, Leblond2 used such assessment for identifying the three major functional types of cellular populationnamely, static, conditional renewal, and continually renewing. The practice of histopathology involves direct or, more usually, indirect assessment of cellular proliferation (and related phenomena such as differentiation) in many situations.3 It is intended that this brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological materialread more
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A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.
TL;DR: The Annexin V assay offers the possibility of detecting early phases of apoptosis before the loss of cell membrane integrity and permits measurements of the kinetics of apoptotic death in relation to the cell cycle.
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Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some, neoplasms
Peter A. Hall,David A. Levison,A. L. Woods,C. C.-W. Yu,D. B. Kellock,J. A. Watkins,Diana M. Barnes,Cheryl Gillett,Richard Camplejohn,Robin Dover,N. H. Waseem,David P. Lane +11 more
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Evidence That Human Cardiac Myocytes Divide after Myocardial Infarction
Antonio Paolo Beltrami,Konrad Urbanek,Jan Kajstura,Shaomin Yan,Nicoletta Finato,Rossana Bussani,Bernardo Nadal-Ginard,Silvestri F,Annarosa Leri,Carlo Alberto Beltrami,Piero Anversa +10 more
TL;DR: The results challenge the dogma that the adult heart is a postmitotic organ and indicate that the regeneration of myocytes may be a critical component of the increase in muscle mass of the myocardium.
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Monoclonal antibody Ki-67: its use in histopathology.
D. C. Brown,K C Gatter +1 more
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In situ end-labelling detects DNA strand breaks in apoptosis and other physiological and pathological states
TL;DR: In situ end‐labelling stains cells with the morphological characteristics of apoptosis, and greatly simplifies their identification, and in two model systems, the number of labelled cells parallels thenumber of cells undergoing apoptosis as measured by alternative techniques.
References
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TL;DR: Assessments of proliferative activity clearly have prognostic potential in centroblastic-centrocytic lymphoma and may permit more accurate characterisation of individual tumours.
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Immunohistochemical determination of growth fractions in human permanent cell lines and lymphoid tumours: A critical comparison of the monoclonal antibodies OKT9 and Ki-67
Stefano Pileri,Johannes Gerdes,M.T. Rivano,Pier Luigi Tazzari,Monica Magnani,Marco Gobbi,Harald Stein +6 more
TL;DR: OKT9 and Ki‐67 monoclonal antibodies have recently been proposed as useful tools for evaluating the growth fraction in malignant tumours, with special reference to non‐1 Hodgkin's lymphomas.
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Histiocytic necrotizing lymphadenitis without granulocytic infiltration (Kikuchi's lymphadenitis). Morphological and immunohistochemical study of eight cases.
M.T. Rivano,Brunangelo Falini,Harald Stein,S. Canino,C. Ciani,Johannes Gerdes,R. Ribacchi,Marco Gobbi,Stefano Pileri +8 more
TL;DR: Eight examples of histiocytic necrotizing lymphadenitis without granulocytic infiltration (Kikuchi's lymphadenopathy) are described, expanding the spectrum of knowledge and allowing speculation as to the biology of this disease.
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The prognostic significance of proliferative activity in poor histology non-Hodgkin's lymphoma: a flow cytometry study using archival material☆
TL;DR: The DNA content and proliferative activity of paraffin-embedded lymph node tissue from 111 patients with poor histology non-Hodgkin's lymphoma were measured by flow cytometry and the incidence of aneuploidy did not correlate with age, stage or survival.