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Journal ArticleDOI

Review: Methods Used to Evaluate the Free Radical Scavenging Activity in Foods and Biological Systems:

Concepción Sánchez-Moreno
- 01 Jun 2002 - 
- Vol. 8, Iss: 3, pp 121-137
TLDR
In this paper, the authors described the methods used for free radical scavenging in both substrates as well as in specific cases of their application, such as superoxide radicals scavenging (O2·-), hydrogen peroxide scavenging(H2O2), hypochlorous acid scavenging, HOCl, and hydroxyl radical scavenges (HO), among them, among them are the methods that use azo-compounds to generate peroxyl radicals.
Abstract
Free radical generation is directly related with oxidation in foods and biological systems. Therefore, the search for methods to determine free radical scavenging is important. In this work are described the methods used for this purpose in both substrates as well as in specific cases of their application. The main methods comprise superoxide radicals scavenging (O2·-); hydrogen peroxide scavenging (H2O2); hypochlorous acid scavenging (HOCl); hydroxyl radical scavenging (HO.); peroxyl radical scavenging (ROO.), among them are the methods that use azo-compounds to generate peroxyl radicals, such as the ``TRAP'' method (Total Radical-Trapping Antioxidant Parameter) and the ``ORAC'' method (Oxygen-Radical Absorbance Capacity); the scavenging of radical cation 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) or the ABTS or the ``TEAC'' method (Trolox Equivalent Antioxidant Capacity); the scavenging of stable radical 2,2-diphenyl-1-picrylhydrazyl or DPPH . method and the scavenging of radical cation N,N-dime...

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Citations
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Journal ArticleDOI

The Chemistry behind Antioxidant Capacity Assays

TL;DR: This analysis suggests that the total phenols assay by FCR be used to quantify an antioxidant's reducing capacity and the ORAC assay to quantify peroxyl radical scavenging capacity, to comprehensively study different aspects of antioxidants.
Journal ArticleDOI

Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements

TL;DR: Methods available for the measurement of antioxidant capacity are reviewed, presenting the general chemistry underlying the assays, the types of molecules detected, and the most important advantages and shortcomings of each method.
Journal Article

The use of the stable free radical diphenylpicrylhydrazyl (dpph) for estimating antioxidant activity

TL;DR: The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity and its application in antioxidant research is described.
Journal ArticleDOI

Free radicals, natural antioxidants, and their reaction mechanisms

TL;DR: The mechanism of action of the natural antioxidant compounds and assays and their reaction mechanisms can help in evaluating the antioxidant activity of various antioxidant compounds as well as in the development of novel antioxidants.
References
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Journal ArticleDOI

Use of a Free Radical Method to Evaluate Antioxidant Activity

TL;DR: The antiradical properties of various antioxidants were determined using the free radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH*) in its radical form as discussed by the authors.
Journal ArticleDOI

Antioxidant activity applying an improved ABTS radical cation decolorization assay.

TL;DR: A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants.
Book ChapterDOI

Role of free radicals and catalytic metal ions in human disease: an overview.

TL;DR: The chapter discusses the metabolism of transition metals, such as iron and copper, and the chelation therapy that is an approach to site-specific antioxidant protection.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI

Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion

TL;DR: In this paper, the autoxidation of soybean oil in a cyclodextrin emulsion system was studied in the presence of an emulsion stabilizer consisting of polysaccharides such as xanthan, tragacanth gum, and methylcellulose.
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