Journal ArticleDOI
RSPS version 4.0: a semi-interactive vector-search program for solving heavy-atom derivatives
TLDR
A program for inspection and interpretation of the Patterson function is described, mainly intended for finding heavy-atom positions from difference Patterson maps, but may also be used to locate molecules with non-crystallographic symmetry when the local axis is nearly parallel to a crystallographic symmetry axis.Abstract:
A program for inspection and interpretation of the Patterson function is described. The program is mainly intended for finding heavy-atom positions from difference Patterson maps, but may also be used to locate molecules with non-crystallographic symmetry when the local axis is nearly parallel to a crystallographic symmetry axis. Options are available for vector-based methods to locate heavy-atom sites, for finding sets from a list of possible heavy-atom positions and for checking of potential solutions. Both crystallographic and non-crystallographic symmetry may be used, either independently or in conjunction.read more
Citations
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Journal ArticleDOI
Crystal structure of a prokaryotic homologue of the mammalian oligopeptide–proton symporters, PepT1 and PepT2
Simon Newstead,David A. Drew,Alexander D. Cameron,Vincent L. G. Postis,Xiaobing Xia,Philip W. Fowler,Jean C. Ingram,Elisabeth P. Carpenter,Mark S.P. Sansom,Michael J. McPherson,Stephen A. Baldwin,So Iwata +11 more
TL;DR: The crystal structure of PepTSo is presented, a functionally similar prokaryotic homologue of the mammalian peptide transporters from Shewanella oneidensis, which reveals a ligand‐bound occluded state for the MFS and provides new insights into a general transport mechanism.
Journal ArticleDOI
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
TL;DR: The crystal structure of a bacterial homologue of ASBT from Neisseria meningitidis (ASBTNM) is reported, which is remarkably similar to the sodium/proton antiporter NhaA, despite having no detectable sequence homology.
Journal ArticleDOI
Alternating access mechanism in the POT family of oligopeptide transporters
Nicolae Solcan,Jane Kwok,Philip W. Fowler,Alexander D. Cameron,Alexander D. Cameron,David A. Drew,So Iwata,Simon Newstead +7 more
TL;DR: Crystallized in an inward open conformation the structure identifies a hinge‐like movement within the C‐terminal half of the transporter that facilitates opening of an intracellular gate controlling access to a central peptide‐binding site, which supports a model for peptide transport that highlights the importance of salt bridge interactions in orchestrating alternating access within the POT family.
Journal ArticleDOI
A two-domain elevator mechanism for sodium/proton antiport.
Chiara Lee,Hae Joo Kang,Christoph von Ballmoos,Simon Newstead,Simon Newstead,Povilas Uzdavinys,David L. Dotson,So Iwata,So Iwata,Oliver Beckstein,Alexander D. Cameron,David A. Drew,David A. Drew +12 more
TL;DR: It is concluded that despite their fast transport rates of up to 1,500 ions per second, Na+/H+ antiporters operate by a two-domain rocking bundle model, revealing themes relevant to secondary-active transporters in general.
Journal ArticleDOI
Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates
David Komander,Alison Fairservice,Maria Deak,Gursant S Kular,Alan R. Prescott,C. Peter Downes,Stephen T. Safrany,Dario R. Alessi,Daan M. F. van Aalten +8 more
TL;DR: Localisation studies suggest that these inositol phosphates serve to anchor a portion of cellular PDK1 in the cytosol, where it could activate its substrates such as p70 S6‐kinase and p90 ribosomal S6 kinase that do not interact with phosphoinositides.
References
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Journal ArticleDOI
The Protein Data Bank: a computer-based archival file for macromolecular structures.
Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
Crystallographic analysis of ribulose 1,5-bisphosphate carboxylase from spinach at 2·4 Å resolution: Subunit interactions and active site
TL;DR: In this paper, the X-ray structure of the quaternary complex of ribulose 1,5-bisphosphate carboxylase/oxygenase from spinach with CO2, Mg2+ and a reaction-intermediate analogue (CABP) has been determined and refined at 2·4 A resolution.
Journal ArticleDOI
ANSIG: A program for the assignment of protein 1H 2D NMR spectra by interactive computer graphics
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