Journal ArticleDOI
Sequence of the Candida albicans gene encoding the secretory aspartate proteinase.
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TLDR
The gene encoding the CandidaAlbicans aspartate proteinase that is secreted by cells grown in protein-containing media was cloned from a C. albicans genomic bank and indicates an amino acid sequence typical of aspartates proteinases, with amino acid sequences homology to other enzymes of this class and a putative signal peptide.Abstract:
The gene encoding the Candida albicans aspartate proteinase that is secreted by cells grown in protein-containing media was cloned from a C. albicans genomic bank. The base sequence of the insert shows a 1173 bp open-reading frame and indicates an amino acid sequence typical of aspartate proteinases, with amino acid sequence homology to other enzymes of this class and a putative signal peptide consisting of 50 amino acids upstream of the active enzyme.read more
Citations
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Molecular and Biotechnological Aspects of Microbial Proteases
TL;DR: Despite the extensive research on several aspects of proteases, there is a paucity of knowledge about the roles that govern the diverse specificity of these enzymes and deciphering these secrets would enable to exploit proteases for their applications in biotechnology.
Journal ArticleDOI
Candida albicans Secreted Aspartyl Proteinases in Virulence and Pathogenesis
TL;DR: Critically discussed the data relevant to each of these seven criteria, with specific emphasis on how this proteinase family could contribute to Candida virulence and pathogenesis.
Journal ArticleDOI
Cell Wall and Secreted Proteins of Candida albicans: Identification, Function, and Expression
TL;DR: Surface mannoproteins are strong immunogens that trigger and modulate the host immune response during candidiasis and may play a role in morphogenesis.
Journal ArticleDOI
Hydrolytic enzymes as virulence factors of Candida albicans.
TL;DR: Prevention and control of Candida infections might be achieved by pharmacological or immunological tools specifically modulated to inhibit virulence factors, e.g. the family of Saps.
Journal ArticleDOI
Candida albicans Cell Wall Proteins
TL;DR: The Candida albicans cell wall maintains the structural integrity of the organism in addtion to providing a physical contact interface with the environment through fibrillar polysaccharides and proteins.
References
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Journal ArticleDOI
Cloning and sequence analysis of cDNA for human cathepsin D.
TL;DR: A high degree of sequence homology was observed between human cathepsin D and other aspartyl proteases, suggesting a conservation of three-dimensional structure in this family of proteins.
Journal ArticleDOI
The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.
TL;DR: A model whereby inactive precursor molecules produced from the PEP4 gene self-activate within the yeast vacuole and subsequently activate other vacuolar hydrolases is proposed.
Journal ArticleDOI
Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.
TL;DR: Analysis of 18 yeast RNA polymerase II promoters suggests that two classes of DNA sequences serve as preferred sites for transcription initiation, and replaced some of the normal CYC-1 start sites with one of these sequences, TCGA, and found that transcription initiates at this newly introduced sequence.
Journal ArticleDOI
Cloning and sequence analysis of cDNA for human renin precursor
Takashi Imai,Hitoshi Miyazaki,Shigehisa Hirose,Hitoshi Hori,Takashi Hayashi,Ryoichiro Kageyama,Hiroaki Ohkubo,Shigetada Nakanishi,Kazuo Murakami +8 more
TL;DR: Close similarities were also observed in the primary structures of renin and aspartyl proteinases that have known three-dimensional structures, suggesting a similar tertiary structure for renin.