Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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TLDR
Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
Citations
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Cultural and Morphological Variability in Rhizoctonia solani Isolates of different hosts of Assam
Manashi Debbarma,Pranab Dutta +1 more
TL;DR: Morphological variability was studied in 6 isolates of R. solani having different hosts from Assam having differenthosts with wide host range.
Journal ArticleDOI
The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains
TL;DR: The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.
Short title: UP-PCR analysis of Rhizoctonia isolates DNA fingerprinting and anastomosis grouping reveal similar genetic diversity in Rhizoctonia species infecting turfgrasses in the transition zone of USA
TL;DR: In this paper, universally primed PCR (UP-PCR) fingerprinting was used to assess genetic diversity of rhizoctonia spp. infecting turfgrasses, which is a common and serious disease of many turfgrass species.
Dissertation
Production of laccase by the phytopathogenic fungus Rhizoctonia solani
TL;DR: This thesis is an investigation into the production of laccases by the phytopathogenic soil fungus Rhizoctonia solani, and the effects of the inhibitory compounds EDTA and SDS on laccase activity was determined.
Journal ArticleDOI
Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA
TL;DR: This study represents the first reported use of rDNA-ITS sequence data to examine AG-4 HG-I and HG-II in P. vulgaris from Turkey and suggests that the Giresun isolates are closely related to isolates obtained from Italy.
References
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Journal ArticleDOI
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Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
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Journal ArticleDOI
Rapid and sensitive protein similarity searches
TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.