Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
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Identification of a New Waitea circinata Variety Causing Basal Leaf Blight of Seashore Paspalum.
TL;DR: The range of potential turfgrass hosts and environmental conditions conducive for disease development suggest that the pathogen may infect other species in addition to seashore paspalum, which is a saline-tolerant, warm-season turfgrass species popular for golf course use in tropical and subtropical climates.
Soil fungi: diversity and detection.
P. Bridge,B. Spooner,D. S. Powlson,Geoffrey L. Bateman,K. G. Davies,J. L. Gaunt,P. R. Hirsch,P. W. Barlow +7 more
TL;DR: In this paper, the authors used a combination of broad spectrum polymerase chain reaction (PCR) detection, coupled with single strand conformation polymorphisms (SSCP) or denaturing gradient gel electrophoresis (DGGE) to detect fungus diversity in soil.
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Identification of candidate pathogenicity determinants of Rhizoctonia solani AG1-IA, which causes sheath blight disease in rice
TL;DR: Key genes and processes that might be crucial for R. solani pathogenesis, including the ability to effectively damage host cell wall and survive in hostile plant environment by managing oxidative stress, cytotoxic compounds, etc, are reported.
Journal ArticleDOI
Characterization and Pathogenicity of Rhizoctonia solani Isolates Affecting Pisum sativum in North Dakota.
Febina M. Mathew,Robin S. Lamppa,Kishore Chittem,Y. W. Chang,M. Botschner,K. Kinzer,Rubella S. Goswami,Samuel G. Markell +7 more
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Studies on anastomosis groups of Rhizoctonia solani isolates causing disease in two forest nurseries in Poland
TL;DR: The dendrogram showed that isolates were distinctly separated based on their AG types and there was no relationship between pathogenicity on Scots pine seedlings and the AG to which the isolates belong to.
References
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Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
TL;DR: Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.
Journal ArticleDOI
Rapid and sensitive protein similarity searches
TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.