Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
Reads0
Chats0
TLDR
Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
Citations
More filters
Journal ArticleDOI
Detection of rDNA ITS polymorphism in Rhizoctonia solani AG 2-1 isolates
Joke Pannecoucque,Monica Höfte +1 more
TL;DR: The sequence variability of the ribosomal internal transcribed spacer regions ITS1 and ITS2, including the 5.8S gene, was investigated for Rhizoctonia solani isolates of anastomosis group (AG) 2-1, and sequences derived from the same isolate did not form distinct clusters, questioning the relevance of further subdivision of heterogeneous AG2-1 isolates based on the ITS region.
Journal ArticleDOI
Genetic diversity, anastomosis groups and virulence of Rhizoctonia spp. from strawberry
TL;DR: This is the first report indicating that Rhizoctonia spp.
Journal ArticleDOI
Identification of Rhizoctonia solani AG 1-IB in lettuce, AG 4 HG-I in tomato and melon, and AG 4 HG-III in broccoli and spinach, in Brazil
TL;DR: Fungi isolated in Brazil, from lettuce, broccoli, spinach, melon and tomato, were identified as Rhizoctonia solani, and DNA sequence analyses of ribosomal internal transcribed spacers showed that isolates from lettuce were AG 1-IB, isolate from tomato and melon were AG 4 HG-I, and isolate from broccoli and spinach were AG4 HG-III.
Journal ArticleDOI
Genetic and genomic analysis of Rhizoctonia solani interactions with Arabidopsis; evidence of resistance mediated through NADPH oxidases
Rhonda C. Foley,Cynthia Gleason,Jonathan P. Anderson,Jonathan P. Anderson,Thorsten Hamann,Karam B. Singh,Karam B. Singh +6 more
TL;DR: Analysis of global gene expression changes in plants infected with AG8 and AG2-1 concluded that reactive oxidative species play an important role in Arabidopsis's resistance to R. solani, and analysed the role of NADPH oxidases in resistance.
Journal ArticleDOI
Development of a specific PCR assay for the detection of Rhizoctonia solani AG 1-IB using SCAR primers.
TL;DR: This study developed a specific and sensitive identification method for Rhizoctonia solani AG 1‐IB isolates based on phylogenetic relationships of R.solani AG‐1 subgroups using rDNA‐internal transcribed spacer (rDNA‐ITS) sequence analysis.
References
More filters
Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
TL;DR: Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.
Journal ArticleDOI
Rapid and sensitive protein similarity searches
TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.