Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
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Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples
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Journal ArticleDOI
THE EVOLUTION OF ASEXUAL FUNGI: Reproduction, Speciation and Classification
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Soil fungi: diversity and detection
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Journal ArticleDOI
Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes.
TL;DR: A primer able to amplify the internal transcribed spacers of the ribosomal DNA (rDNA), having enhanced specificity for ascomycetes, was identified by reviewing fungal ribosomic DNA sequences deposited in GenBank.
Journal ArticleDOI
Hyphal Anastomosis Reactions, rDNA-Internal Transcribed Spacer Sequences, and Virulence Levels Among Subsets of Rhizoctonia solani Anastomosis Group-2 (AG-2) and AG-BI.
TL;DR: Comparing anastomosis reactions, rDNA-internal transcribed spacer (ITS) sequences, and virulence of isolates representing Rhizoctonia solani AG-BI and six subsets of anastsomosis group (AG)-2 found grouping based on virulence does not conform to established grouping patterns within AG-2 and does not seem useful as a group-defining criterion.
References
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Journal ArticleDOI
RFLP analysis of the PCR-amplified 28S rDNA in Rhizoctonia solani
TL;DR: A dendrogram derived from the restriction enzyme data showed that ISGs from AG 1 and AG 2 can each be subdivided into distinct groups, those are distantly related to the majority isolates of the other AGs.