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Journal ArticleDOI

Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani

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TLDR
Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.
Abstract
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.

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Citations
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Journal ArticleDOI

New Anastomose Group of Binucleate Rhizoctonia Causing Leaf Wilt Disease of Erica sp

TL;DR: The pathogen was isolated and purified from diseased leaves, and was identified as binucleate Rhizoctonia sp.

The heterogeneity of the rDNA-ITS sequence and its phylogeny in Rhizoctonia cerealis, the cause of sharp eyespot in wheat

TL;DR: In this paper, the sequence heterogeneity of the ribosomal internal transcribed spacer (ITS) region was investigated for Rhizoctonia cerealis isolates from the anastomosis group AG-DI.
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Genomics of Crucifer’s Host-Pathosystem: Prologue

TL;DR: The genomics of plants was initiated after the sequencing of A. thaliana genome for the first time in 1990 as mentioned in this paper , which has been used widely for dissecting molecular mechanisms of crucifer's host-pathosystem through multi-omics approaches.
References
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Journal ArticleDOI

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Journal ArticleDOI

A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.

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Journal ArticleDOI

Rapid and sensitive protein similarity searches

TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.
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