Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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TLDR
Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
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Rhizoctonia solani: taxonomy, population biology and management of rhizoctonia seedling disease of soybean
TL;DR: This review summarizes what is currently known about the taxonomy and systematics, population biology, and molecular genetics of R. solani and provides an overview of the pathology and management of Rhizoctonia root and hypocotyl rot of soybean, a seedling disease of importance in North America.
Journal ArticleDOI
An evaluation of 18S rDNA approaches for the study of fungal diversity in grassland soils.
TL;DR: The results suggest that 18S rDNA-based approaches are a useful tool for initial screening of fungal communities, and that they represent a more comprehensive picture of the community than plate culturing.
Journal ArticleDOI
High levels of gene flow and heterozygote excess characterize Rhizoctonia solani AG-1 IA (Thanatephorus cucumeris) from Texas.
TL;DR: Data presented here suggest that R. solani AG-1 IA is actively outbreeding (heterothallic) and possible explanations for heterozygote excess, which was observed at all seven RFLP loci, are discussed.
Journal ArticleDOI
Phylogenetic utility of indels within ribosomal DNA and β-tubulin sequences from fungi in the Rhizoctonia solani species complex
TL;DR: P phylogenetic analyses in the Rhizoctonia solani species complex suggest that indels in noncoding regions contain phylogenetic information and support the fact that the R. solANI species complex is not monophyletic.
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Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction.
TL;DR: In this article, the authors developed SYBR Green I-based real-time quantitative-polymerase chain reaction (Q-PCR) assays specific to internal transcribed spacers ITS1 and ITS2 of the nuclear ribosomal DNA of R. solani and R. oryzae.
References
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