Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
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Journal ArticleDOI
Differentiation of Three Homogeneous Groups of Rhizoctonia solani Anastomosis Group 4 by Analysis of Fatty Acids
TL;DR: Fatty acids from 70 isolates of Rhizoctonia solani anastomosis group (AG)-4 clustered into three groups, corresponding to homogeneous group (HG)-I, HG-II, and a newly described HG-III, which is suggested that binomial R. practicola may be appropriate for the portion of AG-4 identified as HG-2.
Journal ArticleDOI
Evolutionary Diversification Indicated by Compensatory Base Changes in ITS2 Secondary Structures in a Complex Fungal Species, Rhizoctonia solani
Paavo Ahvenniemi,Matthias Wolf,Mari J. Lehtonen,P. S. Wilson,Malgorzata German-Kinnari,Jari P. T. Valkonen +5 more
TL;DR: Nine different unambiguous ITS sequences (haplotypes) could be detected in a single strain by sequencing cloned PCR products indicating that concerted evolution had not homogenized the rRNA cistrons in many AG-3 strains.
Journal ArticleDOI
Phylogenetic placement and host specificity of mycorrhizal isolates belonging to AG-6 and AG-12 in the Rhizoctonia solani species complex
Elizabeth J. Pope,Dee A. Carter +1 more
TL;DR: This is the first phylogenetic analysis to include all known AG with a range of host associations and geographic origins including the newly described mycorrhizal AG, and will assist in understanding the evolutionary history and host specificity of this fungus.
Journal ArticleDOI
Can we use environmental DNA as holotypes
Sinang Hongsanan,Rajesh Jeewon,Witoon Purahong,Ning Xie,Jian-Kui Liu,Ruvishika S. Jayawardena,Anusha H. Ekanayaka,Asha J. Dissanayake,Olivier Raspé,Kevin D. Hyde,Marc Stadler,Derek Peršoh +11 more
TL;DR: It is concluded from the evidence that it is highly undesirable to use mgDNA as holotypes in naming fungal species, and an alternative DNA-based system for naming DNA based species is proposed which would provide considerably less problems and should be adopted.
Book ChapterDOI
The impact of molecular data in fungal systematics
TL;DR: Molecular data has had a very significant effect on the understanding of fungal systematics, and many further systematic aspects are likely to be elucidated in the future.
References
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Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
TL;DR: Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.
Journal ArticleDOI
Rapid and sensitive protein similarity searches
TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.