Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
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TLDR
Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.Abstract:
Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.read more
Citations
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Identification and Pathogenicity of Rhizoctonia spp. Causing Wirestem of Betula nigra in China
G.‐H. Yang,S. Naito,W. H. Dong +2 more
TL;DR: During July 2004, wirestem was frequently observed on the seedlings of Betula nigra at Dehong district in Yunnan Province, China, the first report of wirestem of red birch cause by binucleate Rhizoctonia AG-P andAG-R, and R. solani AG-1 IB and AG-4 HG-I in China.
Journal ArticleDOI
Phylogenetic Diversity of Rhizoctonia solani Associated with Canola and Wheat in Alberta, Manitoba, and Saskatchewan
TL;DR: Support is provided for the use of the ITS region as a valuable tool for rapid identification of R. solani isolates to their respective AGs in the phylogenetic analysis.
Journal ArticleDOI
ITS-PCR-RFLP Method for Distinguishing Commercial Cultivars of Edible Mushroom, Flammulina velutipes
TL;DR: Analysis of ribosomal RNA gene regions containing the internal transcribed spacers and the 5.8S rRNA coding sequence revealed the presence of strain-specific deletions, insertions, and substitutions in Flammulina strains.
Journal ArticleDOI
Intraspecific variation of Rhizoctonia solani AG 3 isolates recovered from potato fields in Central Iran and South Australia
G. R. Balali,Stephen M. Neate,A. M. Kasalkheh,Benjamin Stodart,Dara L. Melanson,Eileen S. Scott +5 more
TL;DR: Pectic zymogram, RFLP and PCR analyses were used to characterize Rhizoctonia solani AG 3 isolates collected from diseased potatoes in South Australia and revealed that ZG7-1 generally produced fewer sclerotia and more severe cankers of underground parts of the potato plants than the other two ZGs.
References
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Journal ArticleDOI
Rapid and sensitive protein similarity searches
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