Journal ArticleDOI
Sequencing and modification of psbB, the gene encoding the CP-47 protein of Photosystem II, in the cyanobacterium Synechocystis 6803.
TLDR
The psbB gene, encoding this protein, was cloned from the genome of the cyanobacterium Synechocystis 6803, and sequenced, indicating that an intact CP-47 is required for a functional Photosystem II complex, but does not necessarily indicate that this protein would house the reaction center.Abstract:
The Photosystem II protein CP-47 has been hypothesized to be involved in binding the reaction center chlorophyll. The psbB gene, encoding this protein, was cloned from the genome of the cyanobacterium Synechocystis 6803, and sequenced. The DNA sequence is 68% homologous with that of the psbB gene from spinach, whereas the predicted amino acid sequence is 76% homologous. The hydropathy patterns of Synechocystis and spinach CP-47 are almost indistinguishable, indicating the same general CP-47 folding pattern in the thylakoid membrane in the two species. There are five pairs of histidine residues in CP-47 that are spaced by 13 or 14 amino acids and that are located in hydrophobic regions of the protein; these histidine residues may be involved in chlorophyll binding. Interruption of the psbB gene by a DNA fragment carrying a gene conferring kanamycin resistance results in a loss of Photosystem II activity. This indicates that an intact CP-47 is required for a functional Photosystem II complex, but does not necessarily indicate that this protein would house the reaction center.read more
Citations
More filters
Journal ArticleDOI
Rapid Diversification of Marine Picophytoplankton with Dissimilar Light-Harvesting Structures Inferred from Sequences of Prochlorococcus and Synechococcus (Cyanobacteria)
TL;DR: Standard phylogenetic methods and newer algorithms insensitive to such biases did not recover different branching patterns within the marine picophytoplankton group, and failed to cluster Prochlorococcus with chloroplasts or other chlorophyll b-containing prokaryotes.
Journal ArticleDOI
Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803.
TL;DR: Highly photoactive Photosystem I (PS I) and Photosystem II (PS II) core complexes have been isolated from the cyanobacterium Synechocystis Pasteur Culture Collection (PCC) 6803 and a phycocyanin-deficient mutant, enriched in PS II.
Journal ArticleDOI
The structure and function of CPa-1 and CPa-2 in photosystem II.
TL;DR: Comparisons of the derived amino acid sequences of these proteins suggest sites for chlorophyll binding and for interactions between these chlorophylla-proteins and other Photosystem II components.
Journal ArticleDOI
Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature
Ute Armbruster,Mathias Labs,Mathias Pribil,Stefania Viola,Wen-Teng Xu,Michael Scharfenberg,Alexander Hertle,Ulrike Rojahn,Poul Erik Jensen,Fabrice Rappaport,Pierre Joliot,Peter Dörmann,Gerhard Wanner,Dario Leister,Dario Leister +14 more
TL;DR: It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins, which is evolutionary conserved in plants and cyanobacteria.
Journal ArticleDOI
A High-Resolution Gene Map of the Chloroplast Genome of the Red Alga Porphyra purpurea.
Michael Reith,Janet Munholland +1 more
TL;DR: The increased coding capacity of the P. purpurea chloroplast genome demonstrates the primitive nature of the red alga, and evidence for a monophyletic origin of chloroplasts is suggested by the identification of two groups of genes that are clustered inchloroplast genomes but not in cyanobacteria.
References
More filters
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Generic assignments, strain histories, and properties of pure cultures of cyanobacteria
TL;DR: Revisions are designed to permit the generic identification of cultures, often difficult through use of the field-based system of phycological classification, and are both constant and readily determinable in cultured material.
Journal ArticleDOI
Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution
TL;DR: The molecular structure of the photosynthetic reaction centre from Rhodopseudomonas viridis has been elucidated using X-ray crystallographic analysis and the first description of the high-resolution structure of an integral membrane protein is presented.