Journal ArticleDOI
Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra: Computation of sterically allowed proton-proton distances and statistical analysis of proton-proton distances in single crystal protein conformations
TLDR
Calculated distance measurements imply that corresponding distance measurements can be used for resonance assignments in the side-chains of the aromatic amino acid residues, asparagine and glutamine, where the complete spin systems cannot usually be identified from through-bond spin-spin coupling connectivities.About:
This article is published in Journal of Molecular Biology.The article was published on 1982-03-05. It has received 570 citations till now. The article focuses on the topics: Two-dimensional nuclear magnetic resonance spectroscopy & Resonance.read more
Citations
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Journal ArticleDOI
Pseudo-structures for the 20 common amino acids for use in studies of protein conformations by measurements of intramolecular proton-proton distance constraints with nuclear magnetic resonance.
TL;DR: "Pseudo-structures" of the 20 common amino acid residues are introduced for use in protein spatial structure determinations, which rely on the use of intramolecular proton-proton distance constraints determined by nuclear Overhauser effects as input for distance geometry calculations.
Journal ArticleDOI
Calibration of the angular dependence of the amide proton-Cα proton coupling constants, 3JHNα, in a globular protein: Use of 3JHNα for identification of helical secondary structure
TL;DR: In this paper, the vicinal amide proton-C α proton spin-spin coupling constants, 3 J HNα in the globular protein basic pancreatic trypsin inhibitor (BPTI) have been measured using phase-sensitive correlated spectroscopy at high digital resolution.
Journal ArticleDOI
Conformation and Biological Activity of Cyclic Peptides
TL;DR: In this paper, the authors proposed a model for peptide conformation analysis using NMR spectroscopic methods, including many recent advances, in relation to their ability to contribute to peptide conformational analysis.
Journal ArticleDOI
Polypeptide secondary structure determination by nuclear magnetic resonance observation of short proton-proton distances.
TL;DR: The results obtained imply that the combined information on all these distances obtained from visual inspection of the two-dimensional NOE (NOESY) spectra is sufficient for determination of the helical and beta-sheet secondary structures in small globular proteins.
Journal ArticleDOI
NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231).
TL;DR: The recombinant murine prion protein, mPrP(23-231), was expressed in E. coli with uniform 15N-labeling and NMR experiments showed that the previously determined globular three-dimensional structure of the C-terminal domain mPrp(121-231) is preserved in the intact protein, and that the Nterminal polypeptide segment 23-120 is flexibly disordered as mentioned in this paper, based on nearly complete sequence-specific assignments for the backbone amide nitrogens, amide protons and alpha-protols of
References
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Journal ArticleDOI
The Protein Data Bank: a computer-based archival file for macromolecular structures.
Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
Investigation of exchange processes by two‐dimensional NMR spectroscopy
TL;DR: In this paper, a general technique for the investigation of exchange processes in molecular systems is proposed and demonstrated and applications include the study of chemical exchange, of magnetization transfer by inter-and intramolecular relaxation in liquids, and of spin diffusion and cross-relaxation processes in solids.
Book ChapterDOI
The anatomy and taxonomy of protein structure.
TL;DR: This chapter investigates the anatomy and taxonomy of protein structures, based on the results of three-dimensional X-ray crystallography of globular proteins.
Journal ArticleDOI
Two‐dimensional spectroscopy. Application to nuclear magnetic resonance
TL;DR: In this article, the possibilities for the extension of spectroscopy to two dimensions are discussed, including the elucidation of energy level diagrams, the observation of multiple quantum transitions, and the recording of high-resolution spectra in inhomogenous magnetic fields.
Journal ArticleDOI
Relaxation Processes in a System of Two Spins
TL;DR: Abragam and Pound's method for the calculation of the longitudinal relaxation time was extended to the transverse relaxation time in this paper, which gave rise to a steady and transient Overhauser effect.
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Investigation of exchange processes by two‐dimensional NMR spectroscopy
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