Journal ArticleDOI
Short-lived, small RNAs in the cytoplasm of HeLa cells
TLDR
Two methylated species of mammalian cytoplasmic RNA from HeLa cells are described and appear to be coded by the nuclear genome, as ethidium bromide has little effect on their transcription.About:
This article is published in Cell.The article was published on 1974-09-01. It has received 86 citations till now. The article focuses on the topics: Ethidium bromide & RNA.read more
Citations
More filters
Journal ArticleDOI
Adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection.
TL;DR: Analysis of the mutant's growth defect indicates that the adenovirus VAI RNA is required for efficient translation of viral mRNAs at late times after infection.
Journal ArticleDOI
Monomethylated cap structures facilitate RNA export from the nucleus.
Jörg Hamm,Iain W. Mattaj +1 more
TL;DR: Results suggest that the m7GpppN cap structure is likely to be a signal for RNA export from the nucleus, and that a mutant precursor mRNA unable to form detectable splicing complexes was significantly delayed by incorporation of a hypermethylated cap structure.
Journal ArticleDOI
Small RNA species of the HeLa cell: Metabolism and subcellular localization
Gary Zieve,Sheldon Penman +1 more
TL;DR: The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions and two short-lived cytoplasmic species behave kinetically as precursors to the stable nuclear RNAs.
Journal ArticleDOI
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
TL;DR: The efficiency of trimethylation is reduced by deleting the Sm binding site from U2 RNA, but it is not altered by other mutations in the coding sequence, and the trimethylase activity in Xenopus laevis oocytes is cytoplasmic.
Journal ArticleDOI
Onset of Keratin 17 Expression Coincides with the Definition of Major Epithelial Lineages during Skin Development
TL;DR: It is demonstrated that ectopic lef-1 expression induces K17 protein in the skin of adult transgenic mice, and points to the existence of a molecular relationship between development and wound repair.
References
More filters
Journal ArticleDOI
Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells
TL;DR: Three heterogeneous RNA species in HeLa cells have been characterized with respect to kinetics of labeling, turnover and location, showing that the nucleoplasmic, cytoplasmic and polyribosome-associated messenger fractions are relatively unaffected by doses of actinomycin which completely inhibit ribosomal RNA synthesis.
Journal ArticleDOI
Small molecular weight monodisperse nuclear RNA.
TL;DR: The nuceloplasm and nucleolus of HeLa cells contain six distinct low molecular weight species of RNA that are long-lived and do not appear to be precursor to any cytoplasmic product.
Journal ArticleDOI
Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide.
TL;DR: The synthesis of RNA associated with the mitochondria of HeLa cells is selectively inhibited by relatively low concentrations of ethidium bromide, and the ethidiumsensitive RNA does not appear on membrane-associated polyribosomes.
Journal ArticleDOI
Characterization of a new low molecular weight RNA in HeLa cell ribosomes.
TL;DR: Evidence is presented that 7 s RNA derives from the same polynucleotide precursor as does its accompanying 28s molecule, in contrast to the previously described 5 s rRNA.
Related Papers (5)
Small RNA species of the HeLa cell: Metabolism and subcellular localization
Gary Zieve,Sheldon Penman +1 more