Journal ArticleDOI
Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis
Reads0
Chats0
TLDR
Dot hybridization analysis showed that small previtellogenic oocytes already contain a maximal amount of U1 (and U2) snRNAs, which then decreases to about 20% of that value in fully mature oocytes, even though the cell's volume has increased enormously.About:
This article is published in Cell.The article was published on 1983-02-01. It has received 153 citations till now. The article focuses on the topics: snRNP & SnRNA binding.read more
Citations
More filters
Journal ArticleDOI
Single-step induction of mammary adenocarcinoma in transgenic mice bearing the activated c-neu oncogene
TL;DR: The results indicate that the combination of activated oncogene and tissue context are major determinants of malignant progression and that expression of the activated form of c-neu in the mammary epithelium has particularly deleterious consequences.
Journal ArticleDOI
Major nucleolar proteins shuttle between nucleus and cytoplasm
TL;DR: These unexpected results suggest a role for these major nucleolar proteins in the nucleocytoplasmic transport of ribosomal components and suggest that transient exposure of shuttling proteins to the cy toplasm may provide a mechanism for cytop lasmic regulation of nuclear activities.
Journal ArticleDOI
The SMN–SIP1 Complex Has an Essential Role in Spliceosomal snRNP Biogenesis
TL;DR: It is shown that the SMN-SIP1 complex is associated with spliceosomal snRNAs U1 and U5 in the cytoplasm of Xenopus oocytes, and defects in spliceOSomal snRNP biogenesis may be the cause of SMA.
Journal ArticleDOI
Protein Import into the Cell Nucleus
Colin Dingwall,Ronald A. Laskey +1 more
TL;DR: The study of the mechanisms by which proteins are selectively accumulations in the cell nucleus has lagged behind the study of other transport processes, such as those that direct proteins to the cell exterior or to cytoplasmic organelles.
Journal ArticleDOI
Monomethylated cap structures facilitate RNA export from the nucleus.
Jörg Hamm,Iain W. Mattaj +1 more
TL;DR: Results suggest that the m7GpppN cap structure is likely to be a signal for RNA export from the nucleus, and that a mutant precursor mRNA unable to form detectable splicing complexes was significantly delayed by incorporation of a hypermethylated cap structure.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Related Papers (5)
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus
Michael R. Lerner,Joan A. Steitz +1 more