Journal ArticleDOI
Simple sequence repeat (SSR) markers survey of the cassava (Manihot esculenta Crantz) genome : towards an SSR-based molecular genetic map of cassava
P. Stephenson,Keith J. Edwards,S. Melzer,J. Nkumbira,U. Gullberg,K. Apel,Michael D. Gale,Joe Tohme,Martin A. Fregene +8 more
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TLDR
The development and characterization of 172 new SSR markers for the cassava genome, and the placement of 36 of these markers on the existing RFLP framework map of cassava, confirm SSR as the marker of choice for cassava and demonstrate the transferability of the technology as a low-cost approach to increasing the efficiency ofCassava breeding.Abstract:
The development of PCR-based, easily automated molecular genetic markers, such as SSR markers, are required for realistic cost-effective marker-assisted selection schemes. This paper describes the development and characterization of 172 new SSR markers for the cassava genome. The placement of 36 of these markers on the existing RFLP framework map of cassava is also reported. Two similar enrichment methods were employed. The first method yielded 35 SSR loci, for which primers could be designed, out of 148 putative DNA clones. A total of 137 primer pairs could be designed from 544 putative clones sequenced for the second enrichment. Most of the SSRs (95%) were di-nucleotide repeats, and 21% were compound repeats. A major drawback of these methods of SSR discovery is the redundancy – 20% duplication; in addition, primers could not be designed for many SSR loci that were too close to the cloning site – 45% of the total. All 172 SSRs amplified the corresponding loci in the parents of the mapping progeny, with 66% of them revealing a unique allele in at least one of the parents, and 26% having unique alleles in both of the parents. Of the 36 SSRs that have been mapped, at least 1 was placed on 16 out of the 18 linkage groups of the framework map, indicating a broad coverage of the cassava genome. This preliminary mapping of the 36 markers has led to the joining of a few small groups and the creation of one new group. The abundance of allelic bridges as shown by these markers will lead to the development of a consensus map of the male- and female-derived linkage groups. In addition, the relatively higher number of these allelic bridges, 30% as against 10% for RFLPs in cassava, underscores SSR as the marker of choice for cassava. The 100% primer amplification obtained for this set of primers also confirms the appropriateness of SSR markers for use in cassava genome analysis and the transferability of the technology as a low-cost approach to increasing the efficiency of cassava breeding. Current efforts are geared towards the generation of more SSR markers to attain a goal of 200 SSR markers, or 1 SSR marker every 10 cM.read more
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Development of a genome-wide anchored microsatellite map for common bean ( Phaseolus vulgaris L.)
Matthew W. Blair,Fabio Pedraza,Hector Fabio Buendia,Eliana Gaitán-Solís,Steve Beebe,Paul Gepts,Joe Tohme +6 more
TL;DR: A total of 150 microsatellite markers developed for common beans (Phaseolus vulgaris L) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species as discussed by the authors.
Journal ArticleDOI
Microsatellite repeats in common bean (Phaseolus vulgaris ): isolation, characterization, and cross-species amplification in Phaseolus ssp.
TL;DR: In this article, the authors used microsatellite repeats and established their discriminatory power (D L ) for bean diversity characterization and mapping, and found that microsatellites can be valuable genetic markers for assessing genetic diversity in P. vulgaris.
Journal ArticleDOI
Recombination Difference between Sexes: A Role for Haploid Selection
TL;DR: In plants, heterochiasmy is due to a male-female difference in gametic selection and is not influenced by the presence of heteromorphic sex chromosomes, providing strong empirical support in favour of a population genetic explanation for the evolution of heterochIASmy.
Journal ArticleDOI
Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.)
K. Loridon,Kevin McPhee,Julie Morin,P Dubreuil,Marie-Laure Pilet-Nayel,Grégoire Aubert,Catherine Rameau,Alain Baranger,Clarice J. Coyne,Isabelle Lejeune-Hénaut,Judith Burstin +10 more
TL;DR: This paper aims at providing reliable and cost effective genotyping conditions, level of polymorphism in a range of genotypes and map position of newly developed microsatellite markers in order to promote broad application of these markers as a common set for genetic studies in pea.
Journal ArticleDOI
Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars.
C. He,Vaino Poysa,Kangfu Yu +2 more
TL;DR: The markers developed in this study are primarily from expressed sequences, they can be used not only for molecular mapping, cultivar identification and marker-assisted selection, but for identifying gene-trait relations in tomato.
References
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Journal Article
A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.
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