Journal ArticleDOI
Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR
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TLDR
The overall system proposed, based on an overnight enrichment step followed by DNA isolation and multiplex PCR, was satisfactorily tested for its specificity and sensitivity and allowed the detection of the presence of bacterial DNA and the identification of the target pathogens down to 10 cells/25 g liquid whole egg.About:
This article is published in Food Control.The article was published on 2009-08-01. It has received 122 citations till now. The article focuses on the topics: Nucleic acid amplification technique & Multiplex.read more
Citations
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Rapid detection and identification methods for Listeria monocytogenes in the food chain – A review
TL;DR: A wide variety of culture and alternative methods have been developed in order to detect or quantify this pathogen in food, and here are presented the most rapid and sensitive methods.
Journal ArticleDOI
High resolution melting (HRM) analysis of DNA--its role and potential in food analysis.
TL;DR: The potential of HRM analysis based methods in food analysis, i.e. for the identification of closely related species and cultivars and the Identification of pathogenic microorganisms, is discussed.
Journal ArticleDOI
Development of multiplex loop-mediated isothermal amplification-RFLP (mLAMP-RFLP) to detect Salmonella spp. and Shigella spp. in milk
TL;DR: The mLAMP described here can potentially facilitate simultaneous monitoring of Salmonella and Shigella in a large number of food samples, which could be used as a primary screening method and as a supplement to classical detection method.
Journal ArticleDOI
A multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Bacillus cereus, Vibrio parahaemolyticus, Salmonella spp., Listeria monocytogenes, and Staphylococcus aureus in Korean ready-to-eat food.
TL;DR: The assay was able to specifically simultaneously detect as few as 1 colony-forming unit/mL of each pathogen after enrichment for 12 h, indicating that the developed multiplex PCR assay is an effective and informative supplement for practical use.
Journal ArticleDOI
A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples
Alejandro Garrido,María-José Chapela,Belén Román,Paula Fajardo,Jorge Lago,Juan M. Vieites,Ana G. Cabado +6 more
TL;DR: It is proved that the qPCR method described, including the use of one single enrichment broth, modified TA10, was suitable for the simultaneous and reliable screening of Salmonella spp.
References
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Journal ArticleDOI
Food-related illness and death in the United States.
Paul S. Mead,Laurence Slutsker,Vance Dietz,Linda F. McCaig,Joseph S. Bresee,Craig N. Shapiro,Patricia M. Griffin,Robert V. Tauxe +7 more
TL;DR: Overall, foodborne diseases appear to cause more illnesses but fewer deaths than previously estimated.
Journal ArticleDOI
Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella.
K. Rahn,S.A. De Grandis,R. C. Clarke,Scott A. McEwen,Jorge E. Galán,C C Ginocchio,rd R Curtiss,Carlton L. Gyles +7 more
TL;DR: The detection of 99.4% of Salmonella strains tested and the failure to specifically amplify DNA from non-Salmonellastrains confirm that the invA gene contains sequences unique to Salmoneella and demonstrate that this gene is a suitable PCR target, with potential diagnostic applications.
Tecra UniqueTM Salmonella Test for rapid detection of Salmonella: a comparison between the classical reference method (HRN EN ISO 6579:2002 Microbiology of food and animal feeding stuffs-Horizontal method for the detection of Salmonella spp.) and an alternative rapid method for the detection of Salmonella in food and feed samples
TL;DR: In this paper, uspoređeni rezultati pretraga pet vrsta uzoraka standardnom mikrobioloskom metodom traje od tri do pet dana srezultatima dobivenim Tecra UniqueTM Salmonella testom koji daje re-zultate za 22 sata.
Journal ArticleDOI
Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR.
TL;DR: A protocol enabling simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains was devised and evaluated using artificially contaminated fresh produce to save time and increase the ability to assure food safety.
Journal ArticleDOI
Validation of ISO method 11290 part 2. Enumeration of Listeria monocytogenes in foods.
S L Scotter,S.D Langton,Bertrand Lombard,S.M. Schulten,N Nagelkerke,P.H. in 't Veld,P Rollier,C Lahellec +7 more
TL;DR: The objective was to determine the precision of the method in terms of repeatability (r) and reproducibility (R) using three different food types inoculated with various levels of L. monocytogenes and a typical background flora.