Journal ArticleDOI
Stable expression of immunoglobulin gene V(D)J recombinase activity by gene transfer into 3T3 fibroblasts
David G. Schatz,David Baltimore +1 more
TLDR
It is likely that expression of a single, lymphoid-specific gene in a fibroblast is sufficient to confer V(D)J recombinase activity on that cell.About:Â
This article is published in Cell.The article was published on 1988-04-08. It has received 174 citations till now. The article focuses on the topics: Recombinase activity & Recombinase.read more
Citations
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Journal ArticleDOI
RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
Journal ArticleDOI
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
Yoichi Shinkai,Gary Rathbun,Kong-Peng Lam,E. M. Oltz,Valerie Stewart,Monica Mendelsohn,Jean Charron,Milton Datta,Faith Young,Alan M. Stall,Frederick W. Alt +10 more
TL;DR: Loss of RAG-2 function in vivo results in total inability to initiate V(D)J rearrangement, leading to a novel severe combined immune deficient (SCID) phenotype.
Journal ArticleDOI
RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.
TL;DR: The vast repertoire of immunoglobulins and T cell receptors is generated, in part, by V(D)J recombination, a series of genomic rearrangements that occur specifically in developing lymphocytes.
Journal ArticleDOI
The V(D)J Recombination Activating Gene, RAG-1
TL;DR: The RAG-1 (recombination activating gene-1) genomic locus, which activates V(D)J recombination when introduced into NIH 3T3 fibroblasts, was isolated by serial genomic transfections of oligonucleotide-tagged DNA.
Journal ArticleDOI
Identifying differences in mRNA expression by representational difference analysis of cDNA
M. Hubank,David G. Schatz +1 more
TL;DR: Lisitsyn et al. as discussed by the authors adapted representational difference analysis (RDA) for use with cDNA, which was found to be extremely sensitive, reproducible, and predominantly lacked false positives.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.