Journal ArticleDOI
Structural characterization of the reaction pathway in phosphoserine phosphatase: crystallographic "snapshots" of intermediate states.
Weiru Wang,Ho S. Cho,Rosalind Kim,Jaru Jancarik,Hisao Yokota,Henry H. Nguyen,Igor V. Grigoriev,David E. Wemmer,David E. Wemmer,Sung-Hou Kim,Sung-Hou Kim +10 more
TLDR
High-resolution structures of PSP from Methanococcus jannaschii are presented, which define the open state prior to substrate binding, the complex with phosphoserine substrate bound, and thecomplex with AlF3, a transition-state analog for the phospho-transfer steps in the reaction.About:
This article is published in Journal of Molecular Biology.The article was published on 2002-05-31. It has received 180 citations till now. The article focuses on the topics: Phosphoserine phosphatase & Active site.read more
Citations
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Journal ArticleDOI
Eya protein phosphatase activity regulates Six1–Dach–Eya transcriptional effects in mammalian organogenesis
Xue Li,Kenneth A. Oghi,Jie Zhang,Anna Krones,Kevin T. Bush,Christopher K. Glass,Sanjay K. Nigam,Aneel K. Aggarwal,Richard L. Maas,David W. Rose,Michael G. Rosenfeld +10 more
TL;DR: It is reported that Six1 is required for the development of murine kidney, muscle and inner ear, and that it exhibits synergistic genetic interactions with Eya factors, and it is demonstrated that the Eya family has a protein phosphatase function, and its enzymatic activity is necessary for regulating genes encoding growth control and signalling molecules, modulating precursor cell proliferation.
Journal ArticleDOI
Crystal structure of the calcium pump with a bound ATP analogue.
TL;DR: The crystal structure of the Ca2+ pump of skeletal muscle sarcoplasmic reticulum is described, a representative member of the P-type ATPase superfamily, with an ATP analogue, a Mg2+ and twoCa2+ ions in the respective binding sites.
Journal ArticleDOI
Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues
TL;DR: These structures show that the three cytoplasmic domains rearrange to move six out of ten transmembrane helices, thereby changing the affinity of the Ca2+-binding sites and the gating of the ion pathway.
Journal ArticleDOI
Evolutionary Genomics of the HAD Superfamily: Understanding the Structural Adaptations and Catalytic Diversity in a Superfamily of Phosphoesterases and Allied Enzymes
TL;DR: A comprehensive analysis of the HAD superfamily is presented that identifies their unique structural features and provides a detailed classification of the entire superfamily and suggests that at the highest level the HADS is unified with several other superfamilies, namely the DHH, receiver, von Willebrand A, TOPRIM, classical histone deacetylases and PIN/FLAP nuclease domains.
Journal ArticleDOI
Structural basis of ion pumping by Ca2+-ATPase of the sarcoplasmic reticulum.
TL;DR: In this article, the structures of the Ca2+-ATPase (SERCA1a) have been determined for five different states by X-ray crystallography and the meanings of the rearrangements of the transmembrane helices and those of the cytoplasmic domains as well as the mechanistic roles of phosphorylation are now becoming clear.
References
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Book ChapterDOI
Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
Journal ArticleDOI
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
Axel T. Brunger,Axel T. Brunger,Paul D. Adams,G M Clore,W. L. DeLano,Piet Gros,R.W. Grosse-Kunstleve,R.W. Grosse-Kunstleve,Jiansheng Jiang,J. Kuszewski,Michael Nilges,Navraj S. Pannu,Randy J. Read,Luke M. Rice,Thomas Simonson,Gregory L. Warren +15 more
TL;DR: The Crystallography & NMR System (CNS) as mentioned in this paper is a software suite for macromolecular structure determination by X-ray crystallography or solution nuclear magnetic resonance (NMR) spectroscopy.
Journal ArticleDOI
Improved methods for building protein models in electron density maps and the location of errors in these models.
TL;DR: In this paper, the authors describe strategies and tools that help to alleviate this problem and simplify the model-building process, quantify the goodness of fit of the model on a per-residue basis and locate possible errors in peptide and side-chain conformations.
Journal ArticleDOI
AMoRe: an automated package for molecular replacement
TL;DR: In this paper, a new molecular-replacement package is presented, which is an improvement on conventional methods, based on more powerful algorithms and a new conception that enables automation and rapid solution.
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