Structural Organization of the RNA Polymerase-Promoter Open Complex
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TLDR
The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of Rpo, and permit analysis of effects of a transcriptional activator on theructure of RPo.About:
This article is published in Cell.The article was published on 2000-06-09 and is currently open access. It has received 183 citations till now. The article focuses on the topics: RNA polymerase & Polymerase.read more
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Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
TL;DR: The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 Å resolution and protein–nucleic acid contacts help explain DNA and RNA strand contacts, the specificity of RNA synthesis, “abortive cycling” during transcription initiation, and RNA and DNA translocation during transcription elongation.
Journal ArticleDOI
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution
Dmitry G. Vassylyev,Shun-ichi Sekine,Oleg Laptenko,Jookyung Lee,Marina N. Vassylyeva,Sergei Borukhov,Shigeyuki Yokoyama +6 more
TL;DR: The crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 Å resolution provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
Journal ArticleDOI
Principles of genetic circuit design
TL;DR: In this article, a review describes new tools that aid the construction of genetic circuits and discusses the failure modes encountered when assembling circuits, quantify their impact on performance, and review mitigation efforts.
Principles of genetic circuit design
TL;DR: Better tools, well-characterized parts and a comprehensive understanding of how to compose circuits are leading to a breakthrough in the ability to program living cells for advanced applications, from living therapeutics to the atomic manufacturing of functional materials.
Journal ArticleDOI
Bacterial RNA polymerases: the wholo story
TL;DR: Comparisons with structural analyses of evolutionarily unrelated RNA polymerases reveal unexpected general features of the initiation process of bacterial RNA polymerase holoenzyme.
References
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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes
TL;DR: It is shown how the method can be used to determine the approximate molecular weight of the DNA topoisomerase polypeptide by sectioning a gel on which a partially pure sample has been fractionated by electrophoresis.
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Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
TL;DR: The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees.
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Crystal Structure of Thermus aquaticus Core RNA Polymerase at 3.3 Å Resolution
Gongyi Zhang,Elizabeth A. Campbell,Leonid Minakhin,Catherine Richter,Konstantin Severinov,Seth A. Darst +5 more
TL;DR: The X-ray crystal structure of Thermus aquaticus core RNA polymerase reveals a "crab claw"-shaped molecule with a 27 A wide internal channel that places key functional sites, defined by mutational and cross-linking analysis, on the inner walls of the channel in close proximity to the active center Mg2+.