Structure, Catalysis, and Inhibition of OfChi-h, the Lepidoptera-exclusive Insect Chitinase
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TLDR
Crystal structures of both OfChi-h and its complex with chitoheptaose reveal that OfCha-h possesses a long and asymmetric substrate binding cleft, which is a typical characteristics of a processive exo-chitinase.About:
This article is published in Journal of Biological Chemistry.The article was published on 2017-02-10 and is currently open access. It has received 58 citations till now. The article focuses on the topics: Chitinase & Chitin.read more
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Chitinase: diversity, limitations, and trends in engineering for suitable applications
Ayokunmi Oyeleye,Yahaya M. Normi +1 more
TL;DR: In this review, the variations in features and mechanisms of chitinases that limit their exploitation in biotechnological applications are compiled and recent attempts to engineer chit inases for improved efficiency are highlighted.
Journal ArticleDOI
Glycoside hydrolase family 18 chitinases: The known and the unknown
TL;DR: Research progress on biological function, structural information and inhibition of GH18 chitinases has been reviewed and the remaining uncertainties are highlighted and may facilitate those who intent to develop drugs or agrochemicals based on these enzymes.
Journal ArticleDOI
Co-option of microbial associates by insects and their impact on plant-folivore interactions.
TL;DR: By altering plant detection of herbivory or mediating insect interactions with plant defensive compounds, microbes can effectively improve herbivore performance in real time within and between generations.
Journal ArticleDOI
Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin
Akihiko Nakamura,Akihiko Nakamura,Kei-ichi Okazaki,Tadaomi Furuta,Minoru Sakurai,Ryota Iino,Ryota Iino +6 more
TL;DR: The results demonstrate that SmChiA operates as a burnt-bridge Brownian ratchet wherein the Brownian motion along the single chitin chain is rectified forward by substrate-assisted catalysis.
Journal ArticleDOI
Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects
TL;DR: This work investigates the structure and enzymology of OfChtII, a ChtII derived from the insect pest Ostrinia furnacalis and presents the crystal structures of two catalytically active domains of Of chitinase, both in unliganded form and complexed with chitooligosaccharide substrates.
References
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