Journal ArticleDOI
Studies on the intracellular synthesis of reovirus-specified proteins.
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TLDR
The kinetics of synthesis of capsid polypeptides was studied as a function of the multiplicity of infection and the temperature of incubation and the effect of reovirus infection on host protein synthesis was determined; hostprotein synthesis does not decrease until late during the infection cycle.About:
This article is published in Virology.The article was published on 1970-07-01. It has received 206 citations till now. The article focuses on the topics: Capsid & Protein biosynthesis.read more
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Polypeptide components of virions, top component and cores of reovirus type 3.
TL;DR: The molecular weights of the three size classes of capsid polypeptides are such that they are probably coded by monocistronic messenger RNA molecules transcribed from the L, M, and S segments of reovirus genome RNA.
Journal ArticleDOI
Molecular Mechanisms of Interferon Resistance Mediated by Viral-Directed Inhibition of PKR, the Interferon-Induced Protein Kinase
Michael Gale,Michael G. Katze +1 more
TL;DR: It is speculated that IFN is ineffective as a therapeutic agent against hepatitis C virus because the virus can effectively repress PKR function, and the future directions of this PKR field are discussed.
Journal ArticleDOI
Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes.
W G Dougherty,Bert L. Semler +1 more
TL;DR: The reactions catalyzed by viral proteinases are not simple enzyme-substrate interactions; rather, the processing steps are highly regulated, are coordinated with other viral processes, and frequently involve the participation of other factors.
Journal ArticleDOI
Structure of the Bluetongue Virus Capsid
TL;DR: Seven polypeptides were found to be present in the capsid of the bluetongue virus (BTV), four of which are major and three are minor components, suggesting that different genome segments may be utilized to code for certain capsid proteins.
Journal ArticleDOI
The synthesis of Sendai virus polypeptides in infected cells
Robert A. Lamb,Robert A. Lamb,Brian W. J. Mahy,Brian W. J. Mahy,Purnell W. Choppin,Purnell W. Choppin +5 more
TL;DR: The results of pulse and pulse-chase experiments suggest that large, polyprotein precursors are not involved in Sendai virus replication, and that polypeptides are synthesized from monocistronic messenger RNA species.
References
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Journal ArticleDOI
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
TL;DR: Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation, and the resolution is greater and more detailed than by centrifugations, and many samples can be analysed simultaneously and rapidly.
Journal ArticleDOI
Polypeptide components of virions, top component and cores of reovirus type 3.
TL;DR: The molecular weights of the three size classes of capsid polypeptides are such that they are probably coded by monocistronic messenger RNA molecules transcribed from the L, M, and S segments of reovirus genome RNA.
Journal ArticleDOI
Analysis of C14-labeled proteins by disc electrophoresis
TL;DR: This report presents an alternative procedure involving autoradiography of dried longitudinal gel slices that is relatively uncomplicated and can be used to develop the entire pattern of radioactivity in a gel without significant sacrifice of resolution.
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Hybridization and sedimentation studies on “early” and “late” vaccinia messenger RNA
TL;DR: The pattern of macromolecular biosynthesis during the vaccinia virus multiplication cycle, that is, early and late messenger RNA transcribed, has been studied by means of hybridization and density gradient sedimentation.
Journal ArticleDOI
Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis
TL;DR: Double-stranded ribonucleic acid extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis and calculated that each RNA includes 10 segments.