Journal ArticleDOI
Superoxide and hydrogen peroxide production by Drosophila mitochondria.
TLDR
Superoxide production by Drosophila mitochondria was measured fluorometrically as hydrogen peroxide, using its dependence on substrates, inhibitors, and added superoxide dismutase to determine sites of production and their topology.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2003-10-15. It has received 294 citations till now. The article focuses on the topics: Reactive oxygen species & Superoxide.read more
Citations
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Journal ArticleDOI
How mitochondria produce reactive oxygen species.
TL;DR: The description outlined here facilitates the understanding of factors that favour mitochondrial ROS production and develops better methods to measure mitochondrial O2•− and H2O2 formation in vivo, as uncertainty about these values hampers studies on the role of mitochondrial ROS in pathological oxidative damage and redox signalling.
Journal ArticleDOI
Mitochondrial metabolism of reactive oxygen species.
A. Yu. Andreyev,A. Yu. Andreyev,Yu. E. Kushnareva,Yu. E. Kushnareva,Anatoly A. Starkov,Anatoly A. Starkov +5 more
TL;DR: It is suggested that mitochondria augment intracellular oxidative stress due primarily to failure of their ROS removal systems, whereas the role of mitochondrial ROS emission is yet to be determined and a net increase in mitochondrial ROS production in situ remains to be demonstrated.
Journal ArticleDOI
Targeting Antioxidants to Mitochondria by Conjugation to Lipophilic Cations
TL;DR: In this article, mitochondria-targeted antioxidants have been developed by conjugating the lipophilic triphenylphosphonium cation to an antioxidant moiety, such as ubiquinol or α-tocopherol.
Journal ArticleDOI
Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins.
Martin D. Brand,Charles Affourtit,Telma C. Esteves,Katherine Green,Adrian J. Lambert,Satomi Miwa,Julian L. Pakay,Nadeene Parker +7 more
TL;DR: Observations suggest a hypothesis for the main, ancestral function of uncoupling proteins: to cause mild uncoupled and so diminish mitochondrial superoxide production, hence protecting against disease and oxidative damage at the expense of a small loss of energy.
Journal ArticleDOI
The sites and topology of mitochondrial superoxide production
TL;DR: The relative contribution of each site to mitochondrial reactive oxygen species generation in the absence of electron transport inhibitors is unknown in isolated mitochondria, in cells or in vivo, and may vary considerably with species, tissue, substrate, energy demand and oxygen tension.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
The Free Radical Theory of Aging Matures
Kenneth B. Beckman,Bruce N. Ames +1 more
TL;DR: The status of the free radical theory of aging is reviewed, by categorizing the literature in terms of the various types of experiments that have been performed, which include phenomenological measurements of age-associated oxidative stress, interspecies comparisons, dietary restriction, and the ongoing elucidation of the role of active oxygen in biology.
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High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria.
TL;DR: Inhibition of H2O2 formation by uncoupler, malonate and ADP+Pi is shown to be proportional to the ΔΨ decrease by these compounds, which is in line with the concept that a high proton motive force in state 4 is potentially dangerous for the cell due to an increase in the probability of superoxide formation.
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A mutant Drosophila insulin receptor homolog that extends life-span and impairs neuroendocrine function.
TL;DR: It is concluded that juvenile hormone deficiency, which results from InR signal pathway mutation, is sufficient to extend life-span, and that in flies, insulin-like ligands nonautonomously mediate aging through retardation of growth or activation of specific endocrine tissue.
Journal ArticleDOI
Topology of Superoxide Production from Different Sites in the Mitochondrial Electron Transport Chain
TL;DR: The results do not support the idea that mitochondria produce considerable amounts of reactive oxygen species under physiological conditions and the proportion of electron flow giving rise to hydrogen peroxide with palmitoyl carnitine as substrate is more than an order of magnitude lower than commonly cited values.