Journal ArticleDOI
Synthesis of bacteriophage lambda proteins in vitro.
Raymond F. Gesteland,C. Kahn +1 more
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TLDR
It is shown that λ DNA stimulates a low level of protein synthesis in extracts of E. coli supplemented with RNA polymerase and the main polypeptide product is a low molecular species from the tof operon.Abstract:
λ DNA stimulates a low level of protein synthesis in extracts of E. coli supplemented with RNA polymerase. The synthesis is efficiently and specifically repressed by addition of purified λ repressor. About 90% of the protein product is from the rightwards, or tof, promoter (P R ) and 10% from the leftwards, or N, promoter (P L ). The main polypeptide product is a low molecular species from the tof operon.read more
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Transcription antitermination in vitro by lambda N gene product: Requirement for a phage nut site and the products of host nusA, nusB, and nusE genes
Asis Das,Krystyna Wolska +1 more
TL;DR: It is demonstrated that none of the these nus mutations affects the synthesis of N or the other nus gene products to cause a defect in antitermination, and rather, these mutations have inactivated a set of specific host components, the Nus factors, which are essential for N activity.
Journal ArticleDOI
Synthesis of Diphtheria tox-Gene Products in Escherichia coli Extracts
TL;DR: Preliminary experiments suggest that both toxinogenic and nontoxinogenic strains of the diphtheria bacillus contain a factor that specificially blocks expression of the tox gene.
Journal ArticleDOI
Identification and characterization of the in vitro synthesized gene products of bacteriophage M13.
TL;DR: Results suggest that the latter proteins arise in vivo by cleavage of precursor molecules more than twice as long as their native counterparts present within the virion.
Journal ArticleDOI
Characterization of polypeptides made in vitro from bacteriophage lambda DNA
TL;DR: The results suggest that most of the coupled polypeptide synthesis arises from early regions of λ DNA in vitro, and with a specificity that helped characterize some gene products.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Termination Factor for RNA Synthesis
TL;DR: A new protein has been isolated from E. coli which causes specific termination and release of RNA during synthesis in vitro and has been given the name ρ-factor.
Book
The Bacteriophage Lambda.
TL;DR: No wonder you activities are, reading will be always needed, it is not only to fulfil the duties that you need to finish in deadline time, but also to encourage your mind and thoughts.
Journal ArticleDOI
The operators controlled by the lambda phage repressor.
Mark Ptashne,Nancy Hopkins +1 more