Journal ArticleDOI
Synthetic Human Antibodies: Selecting and Evolving Functional Proteins
Carlos F. Barbas,Jürgen Wagner +1 more
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TLDR
Phage display of antibody fragments provides a convenient format for directed molecular evolution of this biologically important protein architecture and methods have been developed for the mechanism-based selection of catalytic antibodies.About:
This article is published in Methods.The article was published on 1995-10-01. It has received 55 citations till now. The article focuses on the topics: Synthetic antibody & Monoclonal antibody.read more
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Journal ArticleDOI
Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
Anke Krebber,Susanne Bornhauser,Jörg Burmester,Annemarie Honegger,Jörg Willuda,Hans Rudolf Bosshard,Andreas Plückthun +6 more
TL;DR: Several hybridomas whose genes could not be cloned in previous experimental setups, but were successfully obtained with the present system, expressed high amounts of aberrant heavy and light chain mRNAs, which were amplified by PCR and greatly exceeded the amount of binding antibody sequences.
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CDR Walking Mutagenesis for the Affinity Maturation of a Potent Human Anti-HIV-1 Antibody into the Picomolar Range
Wei-Ping Yang,Kimberly Green,Sally Pinz-Sweeney,Amelia T. Briones,Dennis R. Burton,Carlos F. Barbas +5 more
TL;DR: The methodology presented here provides a route for the improvement of the affinities of antibodies typical of tertiary immune responses into the picomolar range and may have profound effects on the utility of antibodies as therapeutic and prophylactic agents.
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Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
Toshiaki Maruyama,Luis L. Rodriguez,Peter B. Jahrling,Anthony Sanchez,Ali S. Khan,Stuart T. Nichol,Clarence J. Peters,Paul W. H. I. Parren,Dennis R. Burton +8 more
TL;DR: The studies indicate that neutralizing antibodies are produced in infection by Ebola virus although probably at a relatively low frequency and the neutralizing antibody may be useful in vaccine design and as a prophylactic agent against Ebola virus infection.
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Chemical Selection for Catalysis in Combinatorial Antibody Libraries
Kim D. Janda,Lee-Chiang Lo,Chih-Hung L. Lo,Mui Mui Sim,Ruo Wang,Chi-Huey Wong,Richard A. Lerner +6 more
TL;DR: A method that allows for the direct chemical selection for catalysis from antibody libraries was devised, whereby the positive aspects of hybridoma technology were preserved and re-formatted in the filamentous phage system to allow direct selection of catalysis.
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Phagemid vectors for phage display: properties, characteristics and construction.
TL;DR: This review summarizes the elements of the phagemids and their corresponding functions and the possible trends and future direction to improve the characteristics of thePhagemid.