Journal ArticleDOI
T Antigen Is Bound to a Host Protein in Sv40-Transformed Cells
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It is reported here that the T antigen in a line of SV40-transformed mouse cells forms an oligomeric complex with a specific cell coded protein.Abstract:
THE early region of the small DNA tumour virus, simian virus 40 (SV40), is known to code for at least two polypeptides, the t and T antigens (‘small t’ and ‘large T’) Both these polypeptides are expressed in cells transformed by the virus1–4, and the T antigen has been shown to be essential for both the initiation and maintenance of the transformed state5–9 We therefore need to know how this T protein interacts with components of the host cell in order to understand the mechanism of SV40-induced transformation We report here that the T antigen in a line of SV40-transformed mouse cells forms an oligomeric complex with a specific cell coded proteinread more
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p53 mutations in human cancers
TL;DR: The p53 mutational spectrum differs among cancers of the colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues as mentioned in this paper.
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Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
Lawrence A. Donehower,Michele Harvey,Betty L. Slagle,Mark J. McArthur,Charles A. Montgomery,Janet S. Butel,Allan Bradley +6 more
TL;DR: Observations indicate that a normal p53 gene is dispensable for embryonic development, that its absence predisposes the animal to neoplastic disease, and that an oncogenic mutant form of p53 is not obligatory for the genesis of many types of tumours.
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The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53
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Participation of p53 Protein in the Cellular Response to DNA Damage
TL;DR: A role for the wild-type p53 protein in the inhibition of DNA synthesis that follows DNA damage is suggested and a new mechanism for how the loss of wild- type p53 might contribute to tumorigenesis is suggested.
Journal ArticleDOI
The p53 tumour suppressor gene
TL;DR: The cell cycle is composed of a series of steps which can be negatively or postively regulated by various factors, chief among the negative regulators is the p53 protein, which can lead to cancer.
References
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Journal Article
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
TL;DR: Bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates.
Journal ArticleDOI
Complete nucleotide sequence of SV40 DNA
Walter Fiers,Roland Contreras,Guy Haegeman,R. Rogiers,A. Van de Voorde,H. Van Heuverswyn,J. Van Herreweghe,Guido Volckaert,M. Ysebaert +8 more
TL;DR: The determination of the total 5,224 base-pair DNA sequence of the virus SV40 has enabled us to locate precisely the known genes on the genome.
Journal ArticleDOI
The genome of simian virus 40
V.B. Reddy,B Thimmappaya,Ravi Dhar,K N Subramanian,B. S. Zain,Julian Pan,Prabhat K. Ghosh,M. L. Celma,Sherman M. Weissman +8 more
TL;DR: The nucleotide sequence of SV40 DNA was determined, and the sequence was correlated with known genes of the virus and with the structure of viral messenger RNA's.
Journal ArticleDOI
Spliced early mRNAs of simian virus 40.
Arnold Berk,Phillip A. Sharp +1 more
TL;DR: Correlation of the structure of two cytoplasmic spliced viral RNAs detected in CV-1 cells during the early phase of simian virus 40 (SV40) infection suggests that expression of the early SV40 genes is partially controlled at the level of splicing of RNAs.
Journal ArticleDOI
Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
TL;DR: Temperature-shift experiments and analysis of SV40 viralDNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun.