Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
TLDR
Temperature-shift experiments and analysis of SV40 viralDNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun.Abstract:
Three temperature-sensitive (ts) mutants of simian virus 40 (SV40) in complementation group A (tsA7, tsA28, tsA30) have been isolated and characterized in permissive and restrictive host cells. At 41 C in the AH line of African green monkey kidney cells, the mutants are deficient in an early function required to produce infectious viral deoxyribonucleic acid (DNA). Temperature-shift experiments and analysis of SV40 viral DNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun. The synthesis of mutant DNA molecules themselves can be initiated by a nonmutant gene product in viral complementation studies at 41 C. The cell, however, cannot substitute a host function to provide the initiator required for the replication of free viral DNA. The viral initiator is also required to establish the stable transformation of 3T3 cells.read more
Citations
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Journal ArticleDOI
T Antigen Is Bound to a Host Protein in Sv40-Transformed Cells
TL;DR: It is reported here that the T antigen in a line of SV40-transformed mouse cells forms an oligomeric complex with a specific cell coded protein.
Journal ArticleDOI
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter
William S. Dynan,Robert Tjian +1 more
TL;DR: Results suggest that direct binding of Sp 1 to sequences in the upstream promoter element is the mechanism by which this factor activates transcription by RNA polymerase II at the SV40 early promoter.
Journal ArticleDOI
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
TL;DR: A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large- T antigen that was detected in the cytoplasm but not the nucleus of cells.
Journal ArticleDOI
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
Gregory Prelich,Cheng-Keat Tan,Matthew Kostura,Michael B. Mathews,Antero G. So,Kathleen M. Downey,Bruce Stillman +6 more
TL;DR: It is shown that PCNA and the polyrnerase-δ auxiliary protein have similar electrophoretic behaviour and are both recognized by anti-PCNA human autoantibodies, and both proteins are functionally equivalent; they stimulate SV40 DNA replication in vitro and increase the processivity of calf thymus DNA polymerase- δ.
Journal ArticleDOI
Die Molekularbiologie der Tumorviren
TL;DR: All classes of vertebrates harbor tumor viruses that are capable of inducing either tumors or leukemias, and these viruses have already been assigned to specific regions of their DNA.
References
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Selective extraction of polyoma DNA from infected mouse cell cultures.
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On the Regulation of DNA Replication in Bacteria
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Enhancement of the Infectivity of Simian Virus 40 Deoxyribonucleic Acid With Diethylaminoethyl-Dextran
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The Vacuolating Virus, S.V.40
B. H. Sweet,Maurice R. Hilleman +1 more
TL;DR: The newly discovered vacuolating virus appears to be a common and essentially ubiquitous contaminant of rhesus monkey kidney cell cultures and a likely common contaminants of cynomolgus kidney cultures in which it develops to high titer without evident cytopathic change.
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The Integrated State of Viral DNA in SV40-Transformed Cells
TL;DR: The physical state of the viral DNA in SV3T3 cells, an SV40-transformed cell line that contains 20 SV40 DNA equivalents per cell, is reported on.