Journal ArticleDOI
The antioxidant effects of vitamin A, C, and E on aflatoxin B1-induced oxidative stress in human lymphocytes.
Reads0
Chats0
TLDR
In conclusion, vitamin A, C, and E exhibited protective effects in human lymphocytes by inhibiting AFB1-induced ROS generation by inhibition of GSH and the activities of superoxide dismutase and GPx and increased level of malondialdehyde.Abstract:
The aim of this study was to investigate the possible protective role of vitamin A, C, and E on aflatoxin B(1)-induced in human lymphocytes using biochemical approaches. The control group received dimethyl sulfoxide, the second group of cultures were administered aflatoxin B(1) (AFB(1)) at a dose of 5 muM. The other group of cultures were treated with AFB(1)+vitamin A (0.5 and 1.0 and 1.5 microM) and AFB(1)+vitamin C (25, 50, and 100 microM) and AFB(1)+vitamin E (40, 100, and 200 microM). The results of this experiment show that AFB(1) significantly decreased the level of GSH and the activities of superoxide dismutase and GPx and increased level of malondialdehyde. Simultaneous supplementation with vitamin A, C, and E restored these parameters to that of normal range. In conclusion, vitamin A, C, and E exhibited protective effects in human lymphocytes by inhibiting AFB(1)-induced ROS generation.read more
Citations
More filters
Journal ArticleDOI
Oxidative stress and oxidative damage in chemical carcinogenesis.
TL;DR: It appears that oxidative stress may both cause as well as modify the cancer process.
Journal ArticleDOI
Antimutagenic compounds and their possible mechanisms of action
TL;DR: The present review attempts to give a brief outline on substances presenting antimutagenic potency and their possible mechanism of action as well as a screening strategy for mutagenicity testing and the characteristics of the most widely used antimUTagenicity assays were described.
Journal ArticleDOI
Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in mice.
Kyung-Chul Choi,W.-T. Chung,Jungkee Kwon,Ji-Yeon Yu,Yong-Suk Jang,S.-M. Park,Seung-Youp Lee,Jeong-Chae Lee +7 more
TL;DR: It is suggested that quercetin does not directly protect against AFB(1)-mediated liver damage in vivo, but exerts a partial role in promoting antioxidative defense systems and inhibiting lipid peroxidation.
Journal ArticleDOI
Mycotoxins and oxidative stress: where are we?
TL;DR: The data associated with oxidative stress as a plausible mechanism for mycotoxin-induced toxicity is summarised and a variety of natural compounds due to their antioxidant capacities have been evaluated.
Journal ArticleDOI
Overview on aflatoxins and oxidative stress
TL;DR: This review presents an overview on aflatoxins and oxidative stress, with an emphasis on the protective role of the antioxidants.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.
Journal ArticleDOI
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase
TL;DR: Glutathione peroxidase activity is found to be associated with a relatively stable, nondialyzable, heat-labile, intracellular component which is separable from hemoglobin, by gel filtration and ammonium sulfate precipitation.
Journal ArticleDOI
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues
TL;DR: The use of the foregoing analytical method in the determination of total and oxidized glutathione contents of rat blood, kidney, and liver gave values in good agreement with those obtained by previous investigators.
Journal ArticleDOI
A simple method for clinical assay of superoxide dismutase.
Yi Sun,L. W. Oberley,Ying Li +2 more
TL;DR: The Cu,ZnSOD concentrations in serum and plasma of patients with cancer of the large intestine tended to be less and greater than these values, respectively, but not statistically significantly so.