Journal ArticleDOI
The cobalt-albumin binding assay: insights into its mode of action.
David Bar-Or,Leonard T. Rael,Raphael Bar-Or,Denetta S. Slone,Charles W. Mains,Nagaraja K. R. Rao,C. Gerald Curtis +6 more
TLDR
The main determinants of the cobalt-HSA binding assay mechanism of action include but are not limited to: the proportion of intact N-terminus of HSA, HSA concentration, plasma cysteine/cystine ratio, plasma pH, and the state of oxidation of cys34 of H SA.About:
This article is published in Clinica Chimica Acta.The article was published on 2008-01-01. It has received 78 citations till now. The article focuses on the topics: Ligand binding assay & Human serum albumin.read more
Citations
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Journal ArticleDOI
Albumin as a zinc carrier: properties of its high-affinity zinc-binding site
TL;DR: Comparisons of X-ray crystal structures of free and fatty-acid bound human serum albumin suggest that zinc binding to this site and fatty acid binding to one of the five major sites may be interdependent, and interactive binding of zinc and long-chain fatty acids to albumin may have physiological implications.
Journal ArticleDOI
A review of the health hazards posed by cobalt.
TL;DR: It might be useful to monitor implant patients for signs of hypothyroidism and polycythemia starting at blood or serum Co concentrations above 100 µg/L, and the likely role of free Co(II) ions to explain dose–response relationships is reviewed.
Journal ArticleDOI
Ischemia modified albumin changes - review and clinical implications.
TL;DR: Ischemia modified albumin (IMA), as measured using the albumin cobalt binding test, is currently the most promising biomarker for early detection of ischemia before the onset of irreversible cardiac injury.
Journal ArticleDOI
Neurotoxicity of cobalt
TL;DR: The aim of this work is to group these cases and to identify a possible mechanism of cobalt neurotoxicity, focusing on hypothetic individual susceptibility such as altered metal-binding proteins, altered transport processes in target cells or polymorphic variation of genetic background.
Journal ArticleDOI
Association between ischemia modified albumin, inflammation and hyperglycemia in type 2 diabetes mellitus.
Michelle Kaefer,Sílvia J. Piva,José A.M. De Carvalho,Dievan Bisognin da Silva,Aline M. Becker,Adriane C. Coelho,Marta M.M.F. Duarte,Rafael Noal Moresco +7 more
TL;DR: Higher levels of IMA and hs-CRP in type 2 diabetes are shown because hyperglycemia and inflammation reduces the capacity of albumin to bind cobalt, resulting in higher IMA levels.
References
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Journal ArticleDOI
A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia-a preliminary report.
TL;DR: In a preliminary clinical study of 139 emergency patients with acute chest pain, 99 patients with evidence of myocardial ischemia had elevated assay levels and a colorimetric assay to measure cobalt-HSA binding and record the results in absorbance units was developed.
Journal ArticleDOI
Glutathione measurement in human plasma. Evaluation of sample collection, storage and derivatization conditions for analysis of dansyl derivatives by HPLC.
Dean P. Jones,Joanne L Carlson,Paula S Samiec,Paul Sternberg,Vino C Mody,Robyn L Reed,Lou Ann S. Brown +6 more
TL;DR: In this paper, the authors developed a procedure to minimize error in sample collection, processing and storage that could contribute to human plasma GSH variation over 10-fold despite the use of apparently valid analytical procedures for GSH measurement.
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Sulfenic acid formation in human serum albumin by hydrogen peroxide and peroxynitrite.
TL;DR: Human serum albumin (HSA), the most abundant protein in plasma, has been proposed to have an antioxidant role and HSA-SOH is proposed to serve as an intermediate in the formation ofLow molecular weight disulfides, which are the predominant plasma form of low molecular weight thiols, and in theformation of mixed HSA disulfide, which is present in approximately 25% of circulating HSA.
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Characterization of the Co2+ and Ni2+ binding amino‐acid residues of the N‐terminus of human albumin
TL;DR: Because the degradation of the susceptible, specific transition metal binding site of HSA may account for the decreased cobalt binding observed during ischemic events, an assay that detects this reduced binding could be useful in the diagnosis of ischemia.
Journal ArticleDOI
Characteristics of an Albumin Cobalt Binding Test for Assessment of Acute Coronary Syndrome Patients: A Multicenter Study
Robert H. Christenson,Show-Hong Duh,Wendy R. Sanhai,Alan H.B. Wu,Verena Holtman,Pennell C. Painter,Elizabeth Branham,Fred S. Apple,Maryann Murakami,Deborah L. Morris +9 more
TL;DR: This exploratory study suggests that the ACB Test has high negative predictive value and sensitivity in the presentation sample for predicting troponin-negative or -positive results 6-24 h later.