Journal ArticleDOI
The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts.
Reads0
Chats0
TLDR
Rotative stress has a significant effect on CAII, FAS, FasL, OSCAR, and TRAP gene expression in osteoclasts.Abstract:
This study was designed to explore the effects of rotative stress on carbonic anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), osteoclast-associated receptor (OSCAR), and tartrate-resistant acid phosphatase (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 cells cultured in medium containing recombinant murine soluble receptor activator of NF-Kβ ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, OSCAR, and TRAP genes in osteoclasts was detected by RT-PCR and Western blot, respectively, after osteoclasts were loaded at various rotative stress strengths and times. No significant differences in mRNA and protein expression were observed between any of the control groups (P > 0.05). Importantly, rotative stress had a significant effect on the mRNA and protein expression of these genes (P < 0.05). We found a negative relationship between rotative stress strength and prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In addition, there was a positive relationship between rotative stress strength and prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in osteoclasts. Based on these results, rotative stress has a significant effect on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts.read more
Citations
More filters
Journal ArticleDOI
Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
Takako Hayakawa,Yoshitaka Yoshimura,Takashi Kikuiri,Mino Matsuno,Tomokazu Hasegawa,Kumu Fukushima,Kenjiro Shibata,Yoshiaki Deyama,Kuniaki Suzuki,Junichiro Iida +9 more
TL;DR: Findings suggested that osteoclastogenesis of macrophages was accelerated when an optimal compressive force was applied, compared with the control group which had no effect.
Journal ArticleDOI
Release from optimal compressive force suppresses osteoclast differentiation.
Masaaki Ikeda,Yoshitaka Yoshimura,Takashi Kikuiri,Mino Matsuno,Tomokazu Hasegawa,Kumu Fukushima,Takako Hayakawa,Hajime Minamikawa,Kuniaki Suzuki,Junichiro Iida +9 more
TL;DR: The findings suggest that release from optimal compressive force suppresses osteoclast differentiation and fusion, which may be important for developing orthodontic treatments.
Journal ArticleDOI
Bioactive glass-ceramic for bone tissue engineering: an in vitro and in vivo study focusing on osteoclasts.
Rayana L. Bighetti-Trevisan,Alann T.P. Souza,Ingrid Wezel Tosin,Natália Pieretti Bueno,Murilo C. Crovace,Marcio Mateus Beloti,A. La Rosa,Emanuela Prado Ferraz +7 more
TL;DR: The results enhanced the understanding of the role of bioactive glass-ceramics in bone repair, and highlighted their role in the modulation of osteoclastic activities and promotion of interactions between bone tissues and biomaterials.
Journal ArticleDOI
The Effects of Baicalein on Osteoclast Differentiation from Bone Marrow Derived Macrophage
Patent
A Composition comprising sappanone A for inhibiting of bone loss
TL;DR: In this paper, a composition for inhibiting bone loss containing sappanone A as an active component was proposed, which significantly inhibited osteoclast differentiation and excessive bone absorption by osteoclasts.
References
More filters
Journal ArticleDOI
Mechanotransduction and the functional response of bone to mechanical strain
TL;DR: Mechanotransduction plays a crucial role in the physiology of many tissues including bone and some hormones may interact with local mechanical signals to change the sensitivity of the sensor or effector cells to mechanical load.
Journal ArticleDOI
Force Generation, Transmission, and Integration during Cell and Tissue Morphogenesis
TL;DR: A new era is opening that emphasizes the similarities of and allows comparisons between distant dynamic biological phenomena because they rely on core machineries that control universal features of cytomechanics.
Journal ArticleDOI
Osteocyte Apoptosis Is Induced by Weightlessness in Mice and Precedes Osteoclast Recruitment and Bone Loss
J. Ignacio Aguirre,Lilian I. Plotkin,Scott A. Stewart,Robert S. Weinstein,A. Michael Parfitt,Stavros C. Manolagas,Teresita Bellido +6 more
TL;DR: It is reported that reduced mechanical forces in the murine model of unloading by tail suspension increases the prevalence of osteocyte apoptosis, followed by bone resorption and loss of mineral and strength.
Journal ArticleDOI
Osteocytes as Dynamic Multifunctional Cells
TL;DR: A novel function now attributed to osteocytes includes regulation of phosphate metabolism, in addition to osteoblasts and osteoclasts, which is also important for bone health.
Journal ArticleDOI
Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts.
Zang Hee Lee,Hong-Hee Kim +1 more
TL;DR: A comprehensive understanding of RANK signaling requires further studies on more complicated issues such as the temporal and spatial pattern of the engagement of signaling molecules and the precise relationship between the signaling pathway and the cellular response.