Journal ArticleDOI
The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats.
TLDR
It is suggested that MO has a protective effect against ischemia/reperfusion injury in rat testes and may affect Shh and HIF-1α signaling pathway.Abstract:
This study was designed to determine the effect of molsidomine (MO), a precursor of nitric oxide (NO) donor, on hypoxia inducible factor alpha (HIF-1α) and Sonic hedgehog (Shh) levels considered to be involved in the development of testes ischemia/reperfusion (I-R) injury. Torsions were created by rotating ipsilateral testes 720° in a clockwise direction for 6 h and 1-h detorsion of the testis was performed. A sham operation was performed in group 1 (control, n = 7). In group 2 (I-R/Untreated, n = 7), following 6 h of unilateral testicular torsion, 1-h detorsion of the testis was performed. No drug was given. In group 3 (I-R/MO), after performing the same surgical procedure as in group 2, a NO donor MO was given at the starting time of reperfusion. In group 4 (I-R/L-NAME), after performing the same surgical procedure as in group 2, L-NAME was given at the starting time of reperfusion. Testes malondialdehyde (MDA) levels were determined as well as examining the testes histologically. Treatment of rats with MO produced a significant reduction in the levels of MDA and histopathological score compared to testes I-R groups. The Sonic hedgehog (Shh) expression in the basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue, were greatly increased in the I-R/MO group compared to groups 1, 2 and 4. Additionally, the HIF-1α expression in the interstitial spaces in testicular tissue were greatly increased in the I-R/MO group. The results suggest that MO has a protective effect against ischemia/reperfusion injury in rat testes and may affect Shh and HIF-1α signaling pathway.read more
Citations
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Journal ArticleDOI
The hypoxic testicle: physiology and pathophysiology.
Juan G. Reyes,Jorge G. Farías,Sebastián Henríquez-Olavarrieta,Eva Madrid,Mario Párraga,Andrea B. Zepeda,Ricardo D. Moreno +6 more
TL;DR: It is suggested that conditions such as germ cell apoptosis and DNA damage are common features in hypoxia and varicocele and testicular torsion and oxidative damage seems to be present in these conditions during the initiation stages of germ cell damage and apoptosis.
Journal ArticleDOI
Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain.
TL;DR: The results indicated that polydatin protected the brain from damage caused by pMCAO, and this effect may be through up- Regulating the expression of Gli1, Ptch1 and SOD1 and down-regulating theexpression of NF-κB, and ameliorating BBB permeability.
Journal ArticleDOI
Microparticles Carrying Sonic Hedgehog Favor Neovascularization through the Activation of Nitric Oxide Pathway in Mice
Tarek Benameur,Raffaella Soleti,Chiara Porro,Ramaroson Andriantsitohaina,Maria Carmen Martinez +4 more
TL;DR: It is suggested that MPsShh+ may contribute to reparative neovascularization after ischemic injury by regulating NO pathway and genes involved in angiogenesis.
Journal ArticleDOI
Contemporary Review of Testicular Torsion: New Concepts, Emerging Technologies and Potential Therapeutics
TL;DR: In this review, the new information published over the past four years regarding testicular torsion is highlighted, including: medical legal issues, etiology and genetics, imaging diagnostics, innovative surgical techniques, management controversies, fertility, and new drug therapies.
Journal ArticleDOI
Sonic Hedgehog Protects Cortical Neurons Against Oxidative Stress
TL;DR: It is demonstrated that activation of SHH signaling protects cortical neurons against oxidative stress and suggest a potential role of SHh for the clinic treatments of brain ischemia and neurodegenerative disorders.
References
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HIF-1: mediator of physiological and pathophysiological responses to hypoxia
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Oxygen sensing and molecular adaptation to hypoxia
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Hedgehog signals regulate multiple aspects of gastrointestinal development.
TL;DR: It is shown that Hedgehog signals are essential for organogenesis of the mammalian gastrointestinal tract and suggest that mutations in members of this signaling pathway may be involved in human gastrointestinal malformations.
Journal ArticleDOI
Sertoli cell signaling by Desert hedgehog regulates the male germline
TL;DR: Examination of the developing testis in different genetic backgrounds suggests that Dhh regulates both early and late stages of spermatogenesis, and loss of Patched expression in Dhh mutants suggests a conservation in the Hedgehog signaling pathway between flies and mice, and indicates that Leydig cells may be the direct target of Dhh signaling.