The gene structure and replication of influenza virus.

TLDR: Evidence for a Host-Cell Nuclear Requirement for primary Transcription for Primary Transcription is found and evidence for overlapping Coding Regions using Diff erent Reading Frames in Viruses is found.

Abstract: PERSPECTIVES AND SUMMARy 468 MORPHOLOGY OF THE INFLUENZA VIRUS PARTICLE (VIRION) 469 GENOME STRUCTURE 471 The Segmented Genome 471 ASSigning Gene Functions to RNA Segments 472 Sequences Common to all RNA Segments 474 THE SEQUENCES OF INFLUENZA VIRUS RNA SEGMENTS 474 RNA Segments 1,2, and 3: Transcriptase-Associated Proteins PBI, PB2, and PA ... 476 RNA Segment 4: the Hemagglutinin (HA) 476 RNA Segment 5: the N uc/eocapsid Protein (N P) 481 RNA Segment 6: the Neuraminidase (N A) 482 RNA Segment 7: the Membrane Protein (M 1) and Nonstructural Protein (M2) ......... 484 RN A Segment 8: Nonstructural Proteins NS1 and NS2 487 Overlapping Coding Regions Using Diff erent Reading Frames in Viruses 489 INFLUENZA VIRUS TRANSCRIPTION AND REPLICATION 490 Evidence for a Host-Cell Nuclear Requirement for Primary Transcription 490 In Vitro Transcription of Influenza Virus mRN As 491 In Vivo Transcription of mRN As 494 Template A( )cRNA Synthesis 495 Virion RN A Synthesis 496 The Control of RNA SyntheSis 496 Spliced mRNAs and Their Controlled Synthesis 497 Subgenomic RN As: A Replication Error Produces Defective Interfering Particles .... 498 The Packaging of Eight RNA Segments 498 CONCLUDING REMARKS 499