Journal ArticleDOI
The kinetics of transfer of plasma amino acids to tissues, and the turnover rates of liver and muscle proteins: The use of continuous infusion technique
JoséC. Gan,Henry Jeffay +1 more
TLDR
Under conditions of intravenous infusion, the plasma amino acid specific activity is maintained constant after a period of about 1 h, and it is calculated that protein catabolism in the normal fed rats contributes approx.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1971-10-19. It has received 39 citations till now. The article focuses on the topics: Amino acid & Protein catabolism.read more
Citations
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Journal ArticleDOI
Inhibition of the Lysosomal Pathway of Protein Degradation in Isolated Rat Hepatocytes by Ammonia, Methylamine, Chloroquine and Leupeptin
TL;DR: Protein degradation in isolated rat hepatocytes was measured as the release of [14C]valine from pre-labelled protein, consistent with the view that the majority of short-lived proteins are degraded by the non-lysosomal pathway(s).
Journal ArticleDOI
Relationship of plasma leucine and α-ketoisocaproate during a L-[1-13C]leucine infusion in man: A method for measuring human intracellular leucine tracer enrichment
Dwight E. Matthews,Dwight E. Matthews,H.P. Schwarz,H.P. Schwarz,R. D. Yang,R. D. Yang,Kathleen J. Motil,Kathleen J. Motil,Vernon R. Young,Vernon R. Young,Dennis M. Bier,Dennis M. Bier +11 more
TL;DR: KIC can be used to estimate intracellular leucine tracer enrichment in man during labeled-leucinetracer experiments without requiring tissue biopsy samples, and the constant nature of the plasma KIC toLeucine 13C enrichment ratio implies that relative changes in leucines kinetics will appear the same under many dietary circumstances regardless of whether plasma leucINE or KIC enrichments are used for the calculations.
Journal ArticleDOI
The Biosynthesis of Rat Serum Albumin VI. INTRACELLULAR TRANSPORT OF ALBUMIN AND RATES OF ALBUMIN AND LIVER PROTEIN SYNTHESIS IN VIVO UNDER VARIOUS PHYSIOLOGICAL CONDITIONS
Theodore Peters,James C. Peters +1 more
TL;DR: The agreement between the results obtained with these independent techniques adds some confidence to the use of the [14C]leucine method and is suggested that the stimulus for increased secretion is a small increase in the quantity of albumin in the microsomal particles.
Journal ArticleDOI
The role of methionine in glutathione biosynthesis by isolated hepatocytes.
Donald J. Reed,Sten Orrenius +1 more
TL;DR: Hematocytes freshly isolated from diethylmaleate-treated rats have been shown to perform net biosynthesis of intracellular glutathione at approximately an in vivo rate.
Journal ArticleDOI
Protein degradation in isolated rat hepatocytes is inhibited by ammonia.
TL;DR: Freshly isolated rat hepatocytes, prepared by collagenase perfusion, exhibit a high rate of endogenous proteolysis which is unaffected by amino acids (added as a physiological mixture), but ammonia appears to inhibit endogenous hepatic protein degradation in a selective manner.
References
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Journal ArticleDOI
On the calculation of "turnover time" and "turnover rate" from experiments involving the use of labeling agents.
TL;DR: Calculations on the quantitative determination of the rate of turnover of a substance and their application to experiments involving the use of labeling agents are given, taking into account loss of the isotopic substance by way of breakdown or transport.
Journal ArticleDOI
Intermediate reactions in protein biosynthesis.
Journal ArticleDOI
Studies of metabolic turnover with tritium as a tracer. V. The predominantly non-dynamic state of body constituents in the rat.
Roy C. Thompson,J.E. Ballou +1 more
Journal Article
The effect of low protein diets on the turn-over rates of serums, liver and muscle proteins in the rat, measured by continuous infusion of L-[14C]lysine.
J. C. Waterlow,J. M. L. Stephen +1 more
Journal ArticleDOI
Origins and metabolism of the intracellular amino acid pools in rat liver and muscle.
Jose C. Gan,Henry Jeffay +1 more
TL;DR: After depletion of the labile liver protein, muscle protein breakdown has increased and supplied 65% of muscle intracellular amino acid pool, which in time maintained the plasma and tissue amino acid levels.
Related Papers (5)
Origins and metabolism of the intracellular amino acid pools in rat liver and muscle.
Jose C. Gan,Henry Jeffay +1 more
Protein turnover in skeletal muscle. I. The measurement of rates of synthesis and catabolism of skeletal muscle protein using (14C)Na2CO3 to label protein.
The effect of low protein diets on the turn-over rates of serums, liver and muscle proteins in the rat, measured by continuous infusion of L-[14C]lysine.
J. C. Waterlow,J. M. L. Stephen +1 more