The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli.
TLDR
Two separate enzymes, which determine resistance to inorganic mercury and organomercurials, have been purified from the plasmid-bearing Escherichia coli strain J53-1(R831), suggesting that the native enzyme is composed of three identical subunits.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-06-25 and is currently open access. It has received 195 citations till now. The article focuses on the topics: Phenylmercuric Acetate & Isoelectric point.read more
Citations
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Bacterial mercury resistance from atoms to ecosystems
TL;DR: How this very mobile and plastic suite of proteins protects host cells from this pervasive toxic metal, what roles it has in the biogeochemical cycling of Hg, and how it has been employed in ameliorating environmental contamination are the subjects of this review.
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Phytoremediation of metals: using plants to remove pollutants from the environment
TL;DR: Phytoremediation uses plants to remove pollutants from the environment and the recent discovery that certain chelating agents greatly facilitate metal uptake by soil-grown plants can make this technology a commercial reality in the near future.
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Biomolecule−Mercury Interactions: Modalities of DNA Base−Mercury Binding Mechanisms. Remediation Strategies
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Biodegradation of Aromatic Compounds by Escherichia coli
TL;DR: The recent progress in the understanding of the fundamentals that govern the degradation of aromatic compounds in E. coli makes this bacterium a very useful model system to decipher biochemical, genetic, evolutionary, and ecological aspects of the catabolism of such compounds.
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Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses.
J B Robinson,O H Tuovinen +1 more
TL;DR: It is shown that under aerobic conditions, methylmercury formation under Anaerobic conditions and under Aerobic conditions is more stable than under either of the other conditions.
References
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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The Determination of Enzyme Dissociation Constants
Hans Lineweaver,Dean Burk +1 more
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
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The gel-filtration behaviour of proteins related to their molecular weights over a wide range
TL;DR: Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5.