Journal ArticleDOI
The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an alpha/beta hydrolase fold.
TLDR
The crystal structure of the bromoperoxidase A2 from Streptomyces aureofaciens (ATCC 10762) has been determined by isomorphous replacement and refined to 2.05 A resolution with an R-value of 18.4%.Abstract:
The crystal structure of the bromoperoxidase A2 from Streptomyces aureofaciens (ATCC 10762) has been determined by isomorphous replacement and refined to 2.05 A resolution with an R-value of 18.4%. The enzyme catalyzes the bromination of organic compounds in the presence of bromide and peroxide. The structure confirms the absence of cofactors such as metal ions or haem groups and shows the general topology of the alpha/beta hydrolase fold. The active centre is at the end of a deep pocket and includes a catalytic triad of Ser 98, Asp 228 and His 257. The active centre is connected by a narrow tunnel to a second pocket on the enzyme surface.read more
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Microbial carboxyl esterases: classification, properties and application in biocatalysis
TL;DR: Esterases represent a diverse group of hydrolases catalyzing the cleavage and formation of ester bonds and are widely distributed in animals, plants and microorganisms and special emphasis is given on their application in organic synthesis for the resolution of racemates and prostereogenic compounds.
Journal ArticleDOI
Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics
Kira S. Makarova,Kira S. Makarova,L. Aravind,Yuri I. Wolf,Roman L. Tatusov,Kenneth W. Minton,Eugene V. Koonin,Michael J. Daly +7 more
TL;DR: An extensive comparative sequence analysis of the Deinococcus genome suggests that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism.
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Anatomy of lipase binding sites: the scissile fatty acid binding site
TL;DR: The model identifies the residues which mediate chain length specificity and thus may guide protein engineering of lipases for changed chain lengthspecificity and was supported by published experimental data on the chainlength specificity profile of various lipases and on mutants of fungal lipases with changed fatty acid chain length Specificity.
Journal ArticleDOI
Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments
TL;DR: A general strategy is described for designing proteins that self assemble into large symmetrical nanomaterials, including molecular cages, filaments, layers, and porous materials, some of which share similar features with natural biological assemblies.
References
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Journal ArticleDOI
Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features
Wolfgang Kabsch,Chris Sander +1 more
TL;DR: A set of simple and physically motivated criteria for secondary structure, programmed as a pattern‐recognition process of hydrogen‐bonded and geometrical features extracted from x‐ray coordinates is developed.
Journal ArticleDOI
Improved methods for building protein models in electron density maps and the location of errors in these models.
TL;DR: In this paper, the authors describe strategies and tools that help to alleviate this problem and simplify the model-building process, quantify the goodness of fit of the model on a per-residue basis and locate possible errors in peptide and side-chain conformations.
Journal ArticleDOI
The Protein Data Bank: a computer-based archival file for macromolecular structures.
Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein
Joel L. Sussman,Michal Harel,Felix Frolow,Christian Oefner,Adrian Goldman,Lilly Toker,Israel Silman +6 more
TL;DR: Modeling of acetylcholine binding to the enzyme suggests that the quaternary ammonium ion is bound not to a negatively charged "anionic" site, but rather to some of the 14 aromatic residues that line the gorge.
Journal ArticleDOI
The α/β hydrolase fold
David L. Ollis,Eong Cheah,Miroslaw Cygler,Bauke W. Dijkstra,Felix Frolow,Sybille M. Franken,Michal Harel,S. Jamse Remington,Israel Silman,Joseph D. Schrag,Joel L. Sussman,Koen H. G. Verschueren,Adrian Goldman +12 more
TL;DR: The alpha/beta hydrolase fold as mentioned in this paper is common to several hydrolytic enzymes of widely differing phylogenetic origin and catalytic function, including the serine protease catalytic triad.