Journal ArticleDOI
The Microbiome of Eucalyptus Roots under Different Management Conditions and Its Potential for Biological Nitrogen Fixation
Eduardo da Silva Fonseca,Raquel S. Peixoto,Alexandre S. Rosado,Fabiano de Carvalho Balieiro,James M. Tiedje,Caio T. C. C. Rachid +5 more
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TLDR
The obtained data suggest that Eucalyptus may benefit from biological nitrogen fixation, with many abundant genera being closely related to nitrogen-fixing bacteria.Abstract:
Eucalyptus plantations offer a cost-effective and renewable source of raw material. There is substantial interest in improving forestry production, especially through sustainable strategies such as the use of plant growth-promoting bacteria. However, little is known about Eucalyptus microbiology. In this study, the endophytic bacterial community was assessed in Eucalyptus urograndis roots using culture-dependent and culture-independent techniques with plants grown under different conditions. Three phyla accounted for approximately 95% of the community, with Actinobacteria corresponding to approximately 59%. This contrasts with previous studies in which Actinobacteria accounted for only 5 to 10%. Our data also revealed a high diversity of bacteria, with 359 different genera but a high level of dominance. Six genera, Mycobacterium, Bradyrhizobium, Streptomyces, Bacillus, Actinospica, and Burkholderia, accounted for more than 50% of the classified sequences. We observed a significant influence of the treatments on some genera, causing changes in the bacterial community structure. The obtained data also suggest that Eucalyptus may benefit from biological nitrogen fixation, with many abundant genera being closely related to nitrogen-fixing bacteria. Using N-depleted media, we also cultured 95 bacterial isolates, of which 24 tested positive for the nifH gene and were able to maintain growth without any N source in the medium.read more
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Plant microbiome–an account of the factors that shape community composition and diversity
Khondoker M. G. Dastogeer,Farzana Haque Tumpa,Afruja Sultana,Mst Arjina Akter,Anindita Chakraborty +4 more
TL;DR: Current insights into the factors driving the assembly of plant microbial communities are discussed and the literature on the driving factors for the composition and community structuring of plant microbiomes is compiled.
Journal ArticleDOI
Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations.
Arthur Prudêncio de Araujo Pereira,Maurício Rumenos Guidetti Zagatto,Carolina B. Brandani,Denise de Lourdes Mescolotti,Simone Raposo Cotta,José Leonardo de Moraes Gonçalves,Elke Jurandy Bran Nogueira Cardoso +6 more
TL;DR: A significant role is suggested for A. mangium in a Eucalyptus plantation to enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations.
Journal ArticleDOI
Mixed Eucalyptus plantations induce changes in microbial communities and increase biological functions in the soil and litter layers
Arthur Prudêncio de Araujo Pereira,Arthur Prudêncio de Araujo Pereira,Ademir Durrer,Thiago Gumiere,José Leonardo de Moraes Gonçalves,Agnès Robin,Jean-Pierre Bouillet,Jun-Tao Wang,Jun-Tao Wang,Jay Prakash Verma,Jay Prakash Verma,Brajesh K. Singh,Elke Jurandy Bran Nogueira Cardoso +12 more
TL;DR: Evidence is provided that changes in the microbial community in mixed A. mangium and E. grandis plantations is correlated with increased C and N cycling, which has implications for increased productivity and environmental sustainability, besides allowing for the optimization of mineral fertilization in forest plantations.
Journal ArticleDOI
Digging deeper to study the distribution of mycorrhizal arbuscular fungi along the soil profile in pure and mixed Eucalyptus grandis and Acacia mangium plantations
Arthur Prudêncio de Araujo Pereira,Maiele Cintra Santana,Joice Andrade Bonfim,Denise de Lourdes Mescolotti,Elke Jurandy Bran Nogueira Cardoso +4 more
TL;DR: It is found that the deep layers, although presenting comparatively low root colonization, still harbour a considerable range of AMF, and some root colonization were present even in the deepest soil layers.
Journal ArticleDOI
Potentials of termite mound soil bacteria in ecosystem engineering for sustainable agriculture
TL;DR: In this paper, the potential of bacteria in termite mound soil to serve as bio-fertilizer and biocontrol as a promising tool for sustainable agriculture is assessed.
References
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Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ nt
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Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
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TL;DR: M mothur is used as a case study to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments.
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An Ordination of the Upland Forest Communities of Southern Wisconsin
Roger H. Bray,John T. Curtis +1 more
TL;DR: It is shown that nature of unit variation is a naajor problenl in systematies, and that whether this variation is diserete, continuous, or in some other form, there is a need for appliGation of (uantitative and statistical methods.
Journal ArticleDOI
Species assemblages and indicator species:the need for a flexible asymmetrical approach
Marc Dufrêne,Pierre Legendre +1 more
TL;DR: A new and simple method to find indicator species and species assemblages characterizing groups of sites, and a new way to present species-site tables, accounting for the hierarchical relationships among species, is proposed.
Book
Nucleic acid techniques in bacterial systematics
TL;DR: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleics acid probes DNA fingerprinting from macromolecules to trees.