The RNA primer synthesized by primase to initiate phage G4 DNA replication.
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TLDR
With phage G4 DNA as template, primase (the dnaG protein) synthesizes a 26- to 29-residue RNA transcript at the origin of replication that contains a hairpin region of one A-U and seven G-C base pairs.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-02-10 and is currently open access. It has received 116 citations till now. The article focuses on the topics: Primase & DnaG.read more
Citations
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Journal ArticleDOI
The single-stranded DNA-binding protein of Escherichia coli.
R R Meyer,P S Laine +1 more
TL;DR: The single-stranded DNA-binding protein (SSB) of Escherichia coli is involved in all aspects of DNA metabolism: replication, repair, and recombination.
Journal ArticleDOI
Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains.
Lee Rowen,Arthur Kornberg +1 more
TL;DR: The sharp specificity of the dnaG protein in choice of template and the utilization of either deoxyribonucleotides or ribonucleoside triphosphates to produce a hybrid piece only a few residues long suggests that the dnG protein previously named RNA polymerase by renamed primase is being replaced by DNA polymerase III holoenzyme.
Book ChapterDOI
DNA Structure and Gene Regulation
Robert D. Wells,Thomas C. Goodman,Wolfgang Hillen,G. T. Horn,Ronald D. Klein,Jacquelynn E. Larson,U.R. Müller,S.K. Neuendorf,N. Panayotatos,S M Stirdivant +9 more
TL;DR: The role of deoxyribonucleic acid (DNA) structure in gene regulation is discussed and the properties and conformations of various DNAs are considered and static structure as well as dynamic transitions are reviewed.
Journal ArticleDOI
Bacteriophage replication modules
Christoph Weigel,Harald Seitz +1 more
TL;DR: This work reviews bacteriophage replication mechanisms and modules based on the long-standing observation that in most known phage genomes the replication genes are arranged as modules.
Journal ArticleDOI
The Interaction between Helicase and Primase Sets the Replication Fork Clock
TL;DR: In this paper, mutant primases that have an altered interaction with DnaB and that direct the synthesis of Okazaki fragments of altered length compared to the wild-type are described.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
TL;DR: The use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides) is described.
Journal ArticleDOI
EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.
Paul Modrich,D Zabel +1 more
TL;DR: Kinetic analysis of the endonuclease with ColE1 DNA as substrate showed that the enzyme obeys Michaelis-Menten kinetics, and results indicate that the rate-limiting step is release of endonuclear from its DNA product.
Journal ArticleDOI
Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains.
Lee Rowen,Arthur Kornberg +1 more
TL;DR: The sharp specificity of the dnaG protein in choice of template and the utilization of either deoxyribonucleotides or ribonucleoside triphosphates to produce a hybrid piece only a few residues long suggests that the dnG protein previously named RNA polymerase by renamed primase is being replaced by DNA polymerase III holoenzyme.
Journal ArticleDOI
dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
J P Bouché,K Zechel,A Kornberg +2 more
TL;DR: The protein responsible for the initiation of conversion of single-stranded phage G4 DNA to the duplex replicative form has been purified approximately 3000-fold and identified with Escherichia coli dnaG gene product, a rifampicin-resistant RNA polymerase of approximately 64,000 daltons.
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dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
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Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains.
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